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Polyamidoamine (PAMAM) dendrimer conjugate specifically activates the A3 adenosine receptor to improve post-ischemic/reperfusion function in isolated mouse hearts.
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PMID:  22039965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: When stimulated by small molecular agonists, the A3 adenosine receptor (AR) mediates cardioprotective effects without inducing detrimental hemodynamic side effects. We have examined pharmacologically the protective properties of a multivalent dendrimeric conjugate of a nucleoside as a selective multivalent agonist for the mouse A3AR.
RESULTS: A PAMAM dendrimer fully substituted by click chemistry on its peripheral groups with 64 moieties of a nucleoside agonist was shown to be potent and selective in binding to the mouse A3AR and effective in cardioprotection in an isolated mouse heart model of ischemia/reperfusion (I/R) injury. This conjugate MRS5246 and a structurally related model compound MRS5233 displayed binding Ki values of 0.04 and 3.94 nM, respectively, and were potent in in vitro functional assays to inhibit cAMP production. A methanocarba (bicyclo[3.1.0]hexane) ring system in place of ribose maintained a North conformation that is preferred at the A3AR. These analogues also contained a triazole linker along with 5'-N-methyl-carboxamido and 2-alkynyl substitution, previously shown to be associated with species-independent A3AR selectivity. Both MRS5233 and MRS5246 (1 and 10 nM) were effective at increasing functional recovery of isolated mouse hearts after 20 min ischemia followed by 45 min reperfusion. A statistically significant greater improvement in the left ventricular developed pressure (LVDP) by MRS5246 compared to MRS5233 occurred when the hearts were observed throughout reperfusion. Unliganded PAMAM dendrimer alone did not have any effect on functional recovery of isolated perfused mouse hearts. 10 nM MRS5246 did not improve functional recovery after I/R in hearts from A3AR gene "knock-out" (A3KO) mice compared to control, indicating the effects of MRS5246 were A3AR-specific.
CONCLUSIONS: Covalent conjugation to a versatile drug carrier enhanced the functional potency and selectivity at the mouse A3AR and maintained the cardioprotective properties. Thus, this large molecular weight conjugate is not prevented from extravasation through the coronary microvasculature.
Authors:
Tina C Wan; Dilip K Tosh; Lili Du; Elizabeth T Gizewski; Kenneth A Jacobson; John A Auchampach
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2011-10-31
Journal Detail:
Title:  BMC pharmacology     Volume:  11     ISSN:  1471-2210     ISO Abbreviation:  BMC Pharmacol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-12-29     Completed Date:  2012-04-09     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100967806     Medline TA:  BMC Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  11     Citation Subset:  IM    
Affiliation:
Department of Pharmacology/Toxicology and the Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine A3 Receptor Agonists / metabolism,  pharmacology*
Animals
Binding, Competitive
Cyclic AMP / metabolism
Dendrimers / metabolism,  pharmacology*
Female
Mice
Mice, Knockout
Receptor, Adenosine A3 / genetics,  physiology
Reperfusion Injury / physiopathology,  prevention & control*
Grant Support
ID/Acronym/Agency:
R01 HL077707/HL/NHLBI NIH HHS; R01HL077707/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Adenosine A3 Receptor Agonists; 0/Dendrimers; 0/PAMAM Starburst; 0/Receptor, Adenosine A3; 60-92-4/Cyclic AMP
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Journal Information
Journal ID (nlm-ta): BMC Pharmacol
ISSN: 1471-2210
Publisher: BioMed Central
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Copyright ©2011 Wan et al; licensee BioMed Central Ltd.
open-access:
Received Day: 28 Month: 8 Year: 2011
Accepted Day: 31 Month: 10 Year: 2011
collection publication date: Year: 2011
Electronic publication date: Day: 31 Month: 10 Year: 2011
Volume: 11First Page: 11 Last Page: 11
ID: 3247180
Publisher Id: 1471-2210-11-11
PubMed Id: 22039965
DOI: 10.1186/1471-2210-11-11

Polyamidoamine (PAMAM) dendrimer conjugate specifically activates the A3 adenosine receptor to improve post-ischemic/reperfusion function in isolated mouse hearts
Tina C Wan1 Email: twan@mcw.edu
Dilip K Tosh2 Email: toshd@niddk.nih.gov
Lili Du1 Email: lidu@mcw.edu
Elizabeth T Gizewski1 Email: egizewsk@mcw.edu
Kenneth A Jacobson2 Email: kajacobs@helix.nih.gov
John A Auchampach1 Email: jauchamp@mcw.edu
1Department of Pharmacology/Toxicology and the Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
2Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0810, USA

Background

Polyamidoamine (PAMAM) dendrimers can serve as biocompatible polymeric nanocarriers of bioactive molecules of interest. They are versatile in that their size, shape and placement of functional groups can be customized for various applications [1-5]. For example, dendrimers have been studied for targeted drug and gene delivery [4-8] or for use as experimental tools to carry markers to targeted cells that express unique receptors [9-11]. Additionally, the attachment of drugs to dendrimers can prevent their premature degradation or even enhance tissue selectivity [1]. Nucleoside derivatives that activate adenosine receptors (ARs) can also be attached to dendrimers, which can greatly improve potency or effectiveness of the nucleoside ligands [12-14].

In the present study, an agonist of the A3AR conjugated to a PAMAM dendrimer was characterized pharmacologically and tested for protective effects in an isolated mouse heart model of ischemia/reperfusion (I/R) injury. The A3AR is one of four subtypes among a G protein-coupled receptor (GPCR) family (A1, A2A, A2B and A3) that responds to extracellular adenosine [15]. Adenosine levels are significantly increased during ischemic stress, thereby activating ARs near the site of injury [16-18]. Of the four subtypes, A1 and A3ARs present in the heart are well known to mediate the cardioprotective effects of adenosine [19-22]. However, unlike the A1AR, when the A3AR is activated by selective agonists, such as Cl-IB-MECA 1 (Figure 1), it does not induce detrimental hemodynamic side effects, such as negative chronotropy, inotropy and dromotropy [20,23-25].

There is a medicinal chemical challenge in designing nucleosides that act as selective A3AR agonists across species. We have reported a class of A3AR agonists that are 5'-N-methyl-carboxamido adenosine analogues containing a methanocarba (bicyclo[3.1.0]hexane) ring system in place of ribose to maintain a preferred North (N) conformation, but some of the analogues such as MRS3558 2 were shown to display >1,000-fold selectivity for the human (h) A3AR but not the mouse (m) and rat A3ARs [26,27]. This is because the (N)-methanocarba ring system is better tolerated at the A1AR in rodents than in humans, thereby, reducing selectivity for rodent A3ARs [26,27]. Thus, these agonists that are otherwise very selective in human are of limited use in rodent models. However, by varying substituents on the N6 and C2 positions in the same structural series, species independent A3AR selectivity was restored, especially in analogues containing a 2-alkynyl (CC) group [27]. In the present study, we have applied the principle of enhancement of mA3AR selectivity of 2-alkynyl analogues to chain extended structures. Specifically, the dialkyne 5 contains a second alkynyl group at the terminal position for conjugation by click chemistry (Cu(I)-catalyzed 1,3-dipolar cycloaddition reaction) to an azido derivatized PAMAM dendrimer 4b to produce a GPCR-Ligand Dendrimer (GLiDe) conjugate. The click precursor 5 also served as an intermediate for the model compound 3. Both 3 and the corresponding multivalent GLiDe conjugate MRS5246 6 were pharmacologically characterized at mouse ARs, and it was determined whether binding and functionality were affected by conjugation. Then, the cardioprotective effects of these monomeric and polymeric A3AR agonists were evaluated utilizing isolated mouse hearts in a Langendorff-perfusion system.


Results
Agonist structures and competition binding

We characterized the mAR binding of a GLiDe conjugate, MRS5246, and an unconjugated, structurally related monomer agonist, MRS5233 3. The GLiDe conjugate MRS5246 was synthesized, as reported [13,14], by covalently conjugating a precursor monomer, MRS5221 5, onto a fourth generation (G4) fully-substituted azido PAMAM dendrimer precursor 4b (Figure 1). The monomeric compound, MRS5233 3, mimicked the structure of the modification of MRS5221 5 after conjugation to the dendrimer. Thus, the Cu(I)-catalyzed click reaction forms a triazole from the combination of an azido group (on the PAMAM) and a terminal alkyne (on the nucleoside).

From competition radioligand binding assays, it was determined that the GLiDe conjugate, MRS5246, and the monomer, MRS5233, both bind with significantly higher affinity to the mA3AR than to the mA1AR by 359- and 169-fold, respectively (Table 1 & Figure 2A and 2B). When comparing the selectivity of these agonists at the mA3AR, the GLiDe conjugate MRS5246 showed a 98.5-fold higher affinity than the monomer MRS5233 (Table 1, Figure 2C).

cAMP functional assays

cAMP assays in HEK293 cells overexpressing recombinant mARs were utilized to determine the potency, expressed as IC50, of MRS5246 and MRS5233. Both MRS5246 and MRS5233 were full agonists and highly selective for the mA3AR in comparison to the mA1AR (Figure 3A and 3B). In fact, MRS5233 at 100 μM was inactive at the mA1AR. When the conjugate MRS5246 was compared to the monomer MRS5233 in activating the A3AR, the conjugate was 200-fold more potent (0.02 nM vs. 4.0 nM) (Figure 3C).

Isolated mouse heart I/R experiments

MRS5246 and MRS5233 were tested for effects on functional improvement in isolated mouse hearts subjected to global ischemia and reperfusion. The agonists were infused into the coronary circulation of the hearts for 10 min immediately before 20 min of global ischemia. The functional recovery parameters (left ventricular developed pressure [LVDP], rate of contraction [dP/dtmax], rate of relaxation [dP/dtmin], and coronary flow [CF; mL/min/g]) of the hearts were assessed throughout reperfusion and after 45 min of reperfusion and compared to those of control hearts.

First, it was confirmed that the nearly fully azide-derivatized PAMAM dendrimer precursor not bearing any nucleoside moieties, MRS5401 4a (Figure 1), did not invoke any functional effects. There was no improvement of functional recovery following I/R after the hearts were exposed to ≤100 nM MRS5401 (Figure 4). However, the monomeric agonist, MRS5233, and GLiDe conjugate, MRS5246, evoked improved functional recovery compared to control in a concentration-dependent manner (Figures 5 and 6). MRS5246 provided marginally better improvement compared to MRS5233 at both 1 and 10 nM. MRS5246 at the lower concentration of 1 nM produced a statistically significant improvement in the recovery of LVDP throughout reperfusion whereas an equivalent concentration of MRS5233 did not. Thus, MRS5246 exhibited greater potency at providing ischemic protection. 10 nM MRS5246 was also infused into hearts from A3KO mice and subjected to I/R. There was no effect on functional improvement in these hearts compared to control hearts (Figure 7).


Discussion

PAMAM dendrimers are biocompatible polymers with extensive and advantageous applications in drug delivery in vivo. Previous data have shown GLiDe conjugates and other nanoconjugates to acquire beneficial pharmacological characteristics, such as increased receptor affinities, increased potency and/or increased selectivity in comparison to monomeric ligands [1-5,9-14]. These nanocarriers may also provide pharmacokinetic and pharmacodynamic advantages, such as impeded metabolic degradation or tissue selectivity [1-5,9-14]. In our case, the drug is not intended for cleavage from the carrier at the site of action. The extracellular intact conjugate holds the relevant biological activity and can benefit from the versatility of the PAMAM dendrimers for further derivatization or structural modification as needed for the biological application.

For the present studies, an adenosine analogue (MRS5233) selective for the hA3AR, but modified to possess improved selectivity for the A3AR in mouse, was characterized in mouse model experiments [27]. This compound was also investigated as a mA3AR agonist pharmacophore for conjugation to a G4 PAMAM dendrimer for the synthesis of the GLiDe conjugate, MRS5246.

It was determined that both agonists, MRS5246 and MRS5233, bind with high affinity to the mA3AR with Ki values of 0.04 and 3.94 nM, respectively. In comparison to other AR subtypes, they are highly selective for the mA3AR. This includes high selectivity over the A1AR, the subtype to which these analogues might be more likely to bind compared to A2A or A2BARs. It was further determined that MRS5233 and MRS5246 are highly potent in in vitro functional assays to inhibit cAMP production via A3AR-induced activation of Gi proteins compared to that of the A1AR. MRS5246 showed an IC50 of 0.02 nM at the mA3AR compared to 577.6 nM at the mA1AR (ratio of 28,880), while MRS5233 resulted in an IC50 of 4.0 nM at the mA3AR and lacked inhibition at the A1AR up to 0.1 mM. Therefore, these compounds promised to be excellent candidates for the study of cardioprotection by A3AR activation in mouse models.

In comparing the activities of the two compounds, MRS5246 possessed a much higher affinity to the mA3AR compared to the monomer, MRS5233, by 98.5-fold. The conjugated MRS5246 was also 200-fold more potent A3AR agonist than the monomer, MRS5233, in an in vitro cAMP assay. These findings are comparable to previous findings with other GLiDe conjugates, which significantly improved affinity and potency over the unconjugated monomeric agonists [13,14,28]. The Ki values of these agonists in binding to hARs have been reported and indicate that 3, 5, and 6 are all A3AR-selective [13,14,27]. For example, the triazole derivative 3 displayed Ki values of 22.3 ± 1.6 nM and 2440 ± 320 nM at the hA3AR and hA2AAR, respectively, and at 10 μM inhibited only 12 ± 4% of the radioligand binding at the hA1AR [13,14]. Thus, the model compound 3 binds to the mA3AR with 5.7-fold greater affinity than to the hA3AR. The conjugate 6 binds to the mA3AR with 3.5-fold greater affinity than to the hA3AR. In comparison to the A1AR, the A3AR selectivity of 6 is slightly greater in the mouse (359-fold) than human (161-fold).

The binding and in vitro cAMP assays were performed under unrestricted solution conditions, with no intervening biological barriers such as would be encountered in vivo. There is limited hindrance in these experiments for the PAMAM dendrimers, which range in size of around ~45 Å in diameter for a G4 dendrimer [29]. This size estimate does not include the addition of the covalently conjugated agonist moieties, which would further increase the diameter. However, in vivo, the GLiDe conjugates must reach the myocardial targets by extravasation from the coronary microvasculature. It has been determined that the extravasation process of PAMAMs alone is dependent on the size and maybe even charge of the compounds [29]. These authors found that with each size-increasing generation of PAMAM dendrimers, up to the G4, there was a weight- and size-dependent decrease in the extravasation of the dendrimers. There was a steady decrease in the rate of extravasation from the G1 to G3 dendrimers, but an especially high decrease between G3 and G4 [29].

However, in light of these intuitive results that greater sizes offer hindrances, the Langendorff isolated heart experiments showed that the GLiDe conjugate, MRS5246, was just as, maybe even more, effective than the monomeric agonist in generating cardioprotection after I/R. MRS5246 and MRS5233 were effective in a concentration-dependent manner, where both showed significant protection starting at one nanomolar, and the effects were greater at 10 nM. The percentage of improvements after 45 min of reperfusion was marginally greater for the GLiDe conjugate at these concentrations. However, the multivalent conjugate MRS5246 (1 nM) was more effective than the monomer MRS5233 in improving LVDP over control when observing the hearts throughout reperfusion. Since we did not obtain a direct measure of cell death, it should be noted that we do not know whether the improvements in functional recovery with agonist treatment observed in the isolated mouse heart studies resulted from protection against reversible injury (i.e., myocardial "stunning") and/or necrotic cell injury (i.e., infarction).


Conclusions

Conjugation of an A3AR-specific agonist to a PAMAM dendrimer indeed greatly improved its in vitro affinity and potency compared to the monomer itself. However, despite the greatly increased size and bulkiness of the GLiDe conjugate, it retained its potent cardioprotective effects in mouse isolated perfused hearts, and it was more effective than its smaller monomeric counterpart. This implied that the barrier for extravasation from the coronary microvasculature did not prevent passage of at least some of the polymeric conjugate.

These findings provide assurance that GLiDe conjugates warrant further study in vivo of their very important advantages over the unconjugated ligands. Because ARs are expressed throughout the body and produce different effects, these specific mA3AR dendrimers can be utilized to investigate methods to specifically target them to the myocardium, perhaps on the basis of an antibody complex. It has been determined that there is enhanced stability of ligands when they are conjugated to PAMAM dendrimers [30]. Protection of A3AR agonists against early degradation in the form of GLiDe conjugates is essential, especially considering the findings that A3AR agonists may be cardioprotective, but some are very short-lived. It is also becoming evident that GPCRs, including ARs, may function more efficiently as homo- or heterodimers [31-33]. These dendrimers, which provide ways to control the dimensions or spacing between the functional groups, will be important for studying ARs as oligomers. Additionally, other GPCR-binding ligands, such as opioids, also provide cardioprotection, in addition to findings that adenosine and opioid receptors can dimerize [34]. These dendrimeric carriers can be utilized to carry multiple agents to targeted tissues as enhanced cardioprotectants.


Methods
Chemical synthesis and reagents

Adenosine agonists 3, 5, and 6 were synthesized as reported [13,14]. Adenosine agonists were first dissolved in DMSO and stored at -20 °C as stock solutions. PSB603 was purchased from Tocris Biosciences (Ellisville, MO), CVT-6883 was obtained from ChemieTek (Indianapolis, IN), and adenosine deaminase (ADA) was from Roche Applied Science (Indianapolis, IN). All other chemicals were from from Sigma-Aldrich (St. Louis, MO).

Radioligand competition assays

The competition assays were performed as previously published [35]. Briefly, to prepare the membranes, HEK293 cells stably expressing recombinant mARs were harvested and homogenized in 10 mM Na+-HEPES buffer (pH 7.4) with 10 mM EDTA and 0.1 mM benzamidine. The cell lysates were centrifuged (35,000 × g for 25 min; 4°C), and the pellets were resuspended in 10 mM Na+-HEPES buffer (pH 7.4) with 1 mM EDTA and 0.1 mM benzamidine. The suspensions were homogenized and centrifuged again (35,000 × g for 25 min; 4°C). The resultant pellets were re-suspended in buffer containing 10% sucrose and stored at -80°C until use.

For the radioligand binding assays, 50 μg cell membranes (100 μL) were incubated in 10 mM Na+-HEPES buffer (pH 7.4) with 5 mM MgCl2, 5 U/mL ADA, and [125I]I-AB-MECA as radioligand. The competing ligands of interest were added at various concentration ranges. The IC50 values were calculated using nonlinear regression analysis by fitting the data to the following equation:


binding=nonspecific binding+total binding-nonspecific binding∕1+10x-logIC50

Inhibition constant (Ki) values were calculated using the Cheng-Prusoff equation [36]. The concentration of MRS5246 reflected the concentration of the dendrimeric complex, not the individual ligand moieties. Therefore, all binding Ki values of this dendrimer are to be considered as Kiapp values.

cAMP functional assays

The assays of cAMP (3',5'-cyclic adenosine monophosphate) were performed utilizing a modified protocol published by Nordstedt and colleagues [37], whereby cAMP content was determined by displacement of [3H]cAMP binding to regulatory subunits of protein kinase A in bovine adrenal extracts. HEK293 cells heterologously expressing the mA3AR were prepared by plating 2.5 × 105 cells per well in 24-well plates and cultured overnight in complete media (DMEM with 10% fetal bovine serum/PenStrep/0.4 mg/mL G418) at 37°C. The cells were washed with DMEM/25 mM HEPES (pH 7.4) followed by incubation in DMEM/25 mM HEPES containing 20 μM Ro 20-1274 (phosphodiesterase inhibitor), 1 U/mL ADA and a combination of two selective A2BAR antagonists (300 nM CVT-6883 and 300 nM PSB603) at 37°C for 15 min. Forskolin (20 μM) to induce cAMP production and the test compounds of interest were then simultaneously added. A concentration range of ligands or dendrimer conjugates was tested in triplicate to inhibit cAMP production via activation of A1 or A3 ARs. After incubation at 37°C for 15 min, the media was aspirated, and the cells were lysed with 200 μL ice cold 0.1 M HCl on a shaker at 4°C for 30 min. The lysates were collected and cAMP levels in the acid extract were determined by competitive binding assays as follows. Each lysate sample and cAMP standard were diluted in 0.1 M HCl and subsequently transferred to polypropylene tubes (100 μL). [3H]cAMP (12,000 cpm/100 μL water/tube) and protein extract from bovine adrenal tissue were then added (240 μg adrenal protein/tube at a concentration of 0.6 μg/μL, in the binding protein buffer: 100 mM Tris-HCl, 250 mM NaCl, 10 mM EDTA, 0.1% 2-mercaptoethanol, pH 8.0), with the extract isolated from bovine adrenal glands as described by Nordstedt, et al. [37] to provide the cAMP binding substrate. The samples were incubated at 4°C for 2.5 hours and harvested through GF/C glass microfiber filters using a cell harvester (Brandel, Gaithersburg, MD) and counted in scintillation cocktail. The results were interpolated from standard curves obtained from known concentrations of cAMP standards.

Animals

All experiments were performed with male mice (10-12 weeks of age, weighing ~25-30 g). Wild-type C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, Me). A3KO mice were generated by embryonic stem cell targeting and genotyped by Southern blotting [38]. A3KO mice used had been transferred to the C57BL/6 genetic background by backcrossing greater than 12 generations. All experiments involving animals were approved by the Institutional Animal Care and Use Committee at the Medical College of Wisconsin and complied with the procedures established by the National Institutes of Health Guide for the Care and Use of Laboratory Animals.

Langendorff-Perfused Isolated Mouse Heart Model of I/R

Isolated mouse heart experiments were performed as described previously [20,23]. In brief, hearts were isolated from C57Bl/6J male mice and cannulated via the aorta and perfused in a retrograde fashion by Krebs-Henseleit buffer. Pressure measurements were obtained via a fluid-filled balloon inserted into the left ventricle via the mitral valve and connected to a pressure transducer. The hearts were immersed in warmed perfusion buffer to maintain the hearts at 37°C, and the balloons were inflated to achieve constant end-diastolic pressures of 5 to 10 mm Hg.

The hearts were perfused for 20 min to achieve stabilization and then paced at 420 beats/min for baseline functional measurements. Global ischemia was achieved by closing an in-line stopcock to stop perfusion for 20 min followed by 45 min of reperfusion by re-opening the stopcock. To examine the effect of the ligands and dendrimer conjugates on functional recovery, the hearts were perfused with buffer containing the indicated concentrations of compounds for 10 min immediately before ischemia.

Data Analysis

All data are reported as means ± S.E.M. Functional recovery data in the isolated heart studies were analyzed by two-way repeated measures ANOVA. If global tests showed a main effect, post-hoc contrasts between treatments were performed with Student's t test for unpaired data with the Bonferroni correction. Left ventricular functional recoveries at 45 min of reperfusion were compared using one-way analysis of variance followed by Student's t test with the Bonferroni correction or an unpaired Student's t test, as appropriate. P < 0.05 was statistically significant.


Abbreviations

ADA: adenosine deaminase; AR: adenosine receptor; cAMP: 3',5'-cyclic adenosine monophosphate; CF: coronary flow; CVT-6883: 3-ethyl-1-propyl-8-(1-(3- trifluoromethylbenzyl)-1H-pyrazol-4-yl)-3,7-dihydropurine-2,6-dione; DMSO: dimethylsulfoxide; DMEM: Dulbecco's modified Eagle's medium; EDTA: ethylenediaminetetraacetic acid; Kiapp, apparent inhibition constant; GLiDe: GPCR-ligand dendrimer; GPCR: G protein-coupled receptor; Cl-IB-MECA: 2-chloro-N6-(3-iodobenzyl)-5'-N-methylcarboxamidoadenosine; HEK: human embryonic kidney; HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; [125I]I-AB-MECA: [125I]4-amino-3-iodobenzyl-5'-N-methylcarboxamidoadenosine; LVDP: left ventricular developed pressure; PAMAM: poly(amidoamine); PSB603: 8-[4-[4-(4-chlorophenzyl)piperazide-1-sulfonyl)phenyl]]-1-propylxanthine.


Authors' contributions

TCW planned and performed the animal experiments and wrote the manuscript. LD and ETG performed pharmacological experiments and contributed to assembly of the manuscript. DLT performed chemical synthesis and contributed to the manuscript. KAJ and JAA conceived of the study, planned the experimental strategy, and wrote the manuscript. All authors read and approved the final manuscript.


Authors' information

KAJ is a Senior Investigator at NIDDK, NIH and is a medicinal chemist. JAA is a Professor of Pharmacology/Toxicology at the Medical College of Wisconsin and is a pharmacologist. The research of both groups is focusing on the roles of adenosine receptors.


Acknowledgements and Funding

This research was supported by the National Institutes of Health (R01HL077707 and the NIDDK Intramural Research Program).


References
Esfand R,Tomalia DA,Poly(amidoamine) (PAMAM) dendrimers: from biomimicry to drug delivery and biomedical applicationsDrug Disc TodayYear: 2001642743610.1016/S1359-6446(01)01757-3
Kaczorowska MA,Cooper HJ,Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groupsJ Am Soc Mass SpectromYear: 2008191312121910.1016/j.jasms.2008.06.01618640055
Kim Y,Klutz AM,Jacobson KA,Systematic investigation of polyamidoamine dendrimers surface-modified with poly(ethylene glycol) for drug delivery applications: synthesis, characterization, and evaluation of cytotoxicityBioconjugate ChemYear: 2008191660167210.1021/bc700483s
Yellepeddi VK,Kumar A,Palakurthi S,Surface modified poly(amido)amine dendrimers as diverse nanomolecules for biomedical applicationsExp Opin Drug DelivYear: 2009683585010.1517/17425240903061251
Yellepeddi VK,Kumar A,Palakurthi S,Biotinylated poly(amido)amine (PAMAM) dendrimers as carriers for drug delivery to ovarian cancer cells in vitroAnticancer ResYear: 2009292933294319661298
Navarro G,De Llarduya CT,Activated and non-activated PAMAM dendrimers for gene delivery in vitro and in vivoNanomedYear: 2009528729710.1016/j.nano.2008.12.007
Navarro G,Maiwald G,Haase R,Rogach AL,Wagner E,de Llarduya CT,Ogris M,Low generation PAMAM dendrimer and CpG free plasmids allow targeted and extended transgene expression in tumors after systemic deliveryJ Control ReleaseYear: 20101469910510.1016/j.jconrel.2010.04.03020438778
Patri AK,Kukowska-Latallo JF,Baker JRJ,Targeted drug delivery with dendrimers: comparison of the release kinetics of covalently conjugated drug and non-covalent drug inclusion complexAdv Drug Deliv ResYear: 2005572203221410.1016/j.addr.2005.09.014
Boswell CA,Eck PK,Regino CA,Bernardo M,Wong KJ,Milenic DE,Choyke PL,Brechbiel MW,Synthesis, characterization, and biological evaluation of integrin αvβ3-targeted PAMAM dendrimersMol PharmaceutYear: 2008552753910.1021/mp800022a
Wängler C,Moldenhauer G,Eisenhut M,Haberkorn U,Mier W,Antibody-dendrimer conjugates: the number, not the size of the dendrimers, determines immunoreactivityBioconjugate ChemYear: 20081481168130
Wängler C,Moldenhauer G,Saffrich R,Knapp EM,Beijer B,Schnolzer M,B. W,Eisenhut M,Haberkorn U,Mier W,PAMAM structure-based multifunctional fluorescent conjugates for improved fluorescent labelling of biomacromoleculesChemistryYear: 2008148116813010.1002/chem.20080032818752247
Kecskes A,Tosh DK,Wei Q,Gao ZG,Jacobson KA,GPCR ligand dendrimer (GLiDe) conjugates: adenosine receptor interactions of a series of multivalent xanthine antagonistsBioconjugate ChemYear: 2011221115112710.1021/bc1005812
Tosh DK,Chinn M,Ivanov AA,LKlutz AM,Gao ZG,Jacobson KA,Functionalized congeners of A3 adenosine receptor-selective nucleosides containing a bicyclo[3.1.0]hexane ring systemJ Med ChemYear: 2009527580759210.1021/jm900426g19499950
Tosh DK,Chinn M,Yoo LS,Kang DW,Luecke H,Gao ZG,Jacobson KA,2-Dialkynyl derivatives of (N)-methanocarba nucleosides: 'Clickable' A3 adenosine receptor-selective agonistsBioorgan Med Chem LetYear: 20101850851710.1016/j.bmc.2009.12.018
Fredholm BB,IJzerman AP,Jacobson KA,Linden J,Muller CE,International Union of Basic and Clinical Pharmacology. LXXXI. Nomenclature and classification of adenosine receptors--an updatePharmacol RevYear: 20116313410.1124/pr.110.00328521303899
Auchampach JA,Bolli R,Adenosine receptor subtypes in the heart: therapeutic opportunities and challengesAm J PhysiolYear: 19992763 Pt 2H1113111610070100
Ely SW,Berne RM,Protective effects of adenosine in myocardial ischemiaCirculationYear: 19928538939041537125
Headrick JP,Lasley RD,Adenosine receptors and reperfusion injury of the heartHand Exp PharmacolYear: 200919318921410.1007/978-3-540-89615-9_7
Dougherty C,Barucha J,Schofield PR,Jacobson KA,Liang BT,Cardiac myocytes rendered ischemia resistant by expressing the human adenosine A1 or A3 receptorFASEB JYear: 199812178517929837869
Ge ZD,Peart JN,Kreckler LM,Wan TC,Jacobson MA,Gross GJ,Auchampach JA,Cl-IB-MECA [2-chloro-N6-(3-iodobenzyl)adenosine-5'-N-methylcarboxamide] reduces ischemia/reperfusion injury in mice by activating the A3 adenosine receptorJ Pharmacol Exper TherYear: 20061912001210
Maddock HL,Mocanu MM,Yellon DM,Adenosine A3 receptor activation protects the myocardium from reperfusion/reoxygenation injuryAm J PhysiolYear: 2002283H1307H1313
Tracey WR,Magee WP,Oleynek JJ,Hill RJ,Smith AH,Flynn DM,Knight DR,Novel N6-substituted adenosine 5'-N-methyluronamides with high selectivity for human adenosine A3 receptors reduce ischemic myocardial injuryAm J PhysiolYear: 2003285H2780H2787
Wan TC,Ge ZD,Tampo A,Mio Y,Bienengraeber MW,Tracey WR,Gross GJ,Kwok WM,Auchampach JA,The A3 adenosine receptor agonist CP-532,903 [N6-(2,5-dichlorobenzyl)-3'-aminoadenosine-5'-N-methylcarboxamide] protects against myocardial ischemia/reperfusion injury via the sarcolemmal ATP-sensitive potassium channelJ Pharmacol Exper TherYear: 200732423424310.1124/jpet.107.127480
Auchampach JA,Ge ZD,Wan TC,Moore J,Gross GJ,A3 adenosine receptor agonist IB-MECA reduces myocardial ischemia-reperfusion injury in dogsAm J PhysiolYear: 20032852H607613
Auchampach JA,Rizvi A,Qiu Y,Tang XL,Maldonado C,Teschner S,Bolli R,Selective activation of A3 adenosine receptors with N6-(3-iodobenzyl)adenosine-5'-N-methyluronamide protects against myocardial stunning and infarction without hemodynamic changes in conscious rabbitsCirc ResYear: 19978068008099168782
Jacobson KA,Ji X,Li AH,Melman N,Siddiqui MA,Shin KJ,Marquez VE,Ravi RG,Methanocarba analogues of purine nucleosides as potent and selective adenosine receptor agonistsJ Med ChemYear: 2000432196220310.1021/jm990596510841798
Melman A,Gao ZG,Kumar D,Wan TC,Gizewski ET,Auchampach JA,Jacobson KA,Design of (N)-methanocarba adenosine 5'-uronamides as species-independent A3 receptor-selective agonistsBioorg Med Chem LettYear: 2008182813281910.1016/j.bmcl.2008.04.00118424135
Chanda N,Shukla R,Katti KV,Kannan R,Gastrin releasing protein receptor specific gold nanorods: Breast and prostate tumor avid nanovectors for molecular imagingNANO LetYear: 200991798180510.1021/nl8037147
El-Sayed M,Kiani M,Naimark M,Hikal AH,Ghandehari H,Extravasation of poly(amidoamine) (PAMAM) dendrimers acriss microvascular network endotheliumPharmaceut ResYear: 200118232810.1023/A:1011066408283
Hill E,Shukla R,Park SS,Baker JRJ,Synthetic PAMAM-RGD conjugates target and bind to odontoblast-like MDPC 23 cells and the predentin in tooth organ culturesBioconjugate ChemYear: 2007181756176210.1021/bc0700234
Barnes PJ,Receptor heterodimerization: a new level of cross-talkJ Clin InvestYear: 20061161210121210.1172/JCI2853516670762
Canals M,Burgueño J,Marcellino D,Cabello N,Canela EL,Mallol J,Agnati L,Ferré S,Bouvier M,Fuxe K,et al. Homodimerization of adenosine A2A receptors: qualitative and quantitative assessment by fluorescence and bioluminescence energy transferJ NeurochemYear: 20048872673414720222
Ciruela F,Escriche M,Burgueno J,Angulo E,Cassado V,Soloviev MM,Canela EL,Mallol J,Chan WY,Lluis C,et al. Metabotropic glutamate 1α and adenosine A1 receptors assemble into functionally interacting complexesJ Biol ChemYear: 2001276183451835110.1074/jbc.M00696020011278325
Peart JN,Goss GJ,Cross-talk between adenosine and opioid receptorsDrug News PerspectYear: 20051823724210.1358/dnp.2005.18.4.90865716034479
Auchampach JA,Gizewski ET,Wan TC,de Castro S,Brown GGJ,Jacobson KA,Synthesis and pharmacological characterization of [125I]MRS5127, a high affinity, selective agonist radioligand for the A3 adenosine receptorBiochem PharmacolYear: 20107996797310.1016/j.bcp.2009.11.00919917269
Cheng Y,Prusoff WH,Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reactionBiochem PharmacolYear: 1973223099310810.1016/0006-2952(73)90196-24202581
Nordstedt C,Fredholm BB,A modification of a protein-binding method for rapid quantification of cAMP in cell-culture supernatants and body fluidAnalyt BiochemYear: 199018923123410.1016/0003-2697(90)90113-N2177960
Salvatore CA,Tilley SL,Latour AM,Fletcher DS,Koller BH,Jacobson MA,Disruption of the A3 adenosine receptor gene in mice and its effect on stimulated inflammatory cellsJ Biol ChemYear: 200027564429443410.1074/jbc.275.6.442910660615

Figures

[Figure ID: F1]
Figure 1 

Chemical structures of nucleoside and dendrimer derivatives. MRS5221 5 is a 5'-N-methyl-carboxamido adenosine analogue with a (N)-methanocarba ring system that served as a synthetic precursor for the conjugation to an azide-functionalized G4 PAMAM dendrimer 4b to synthesize the GLiDe conjugate MRS5246 6. The monomer, MRS5233 3 contained a 2-alkynyl chain similar to MRS5221, but also contains a triazole linker group present in the PAMAM conjugate 6. MRS5401 4a is a closely related unliganded PAMAM precursor molecule.



[Figure ID: F2]
Figure 2 

Competition binding assays. Increasing concentrations of MRS5246 or MRS5322 were added to compete for binding with [125I]I-AB-MECA to determine binding affinity, expressed as Ki or Kiapp, to recombinant mARs on HEK293 cell membranes. A & B, Both MRS5246 and MRS5322 bind to the mA3AR with much higher affinity than to the mA1AR. C, binding curves from mA3ARs to compare and show MRS5246 binds with much higher affinity than MRS5322 at the mA3AR.



[Figure ID: F3]
Figure 3 

cAMP functional assays. Increasing concentrations of MRS5246 or MRS5322 were added to activate mA1 or mA3ARs to inhibit forskolin-stimulated cAMP production. A & B, both compounds are much more potent at the mA3AR than at the mA1AR. C, curves from mA3ARs to compare and show MRS5246 was much more potent than MRS5233 at the mA3AR.



[Figure ID: F4]
Figure 4 

PAMAM control dendrimer, MRS5401, alone did not influence functional recovery of isolated mouse hearts subjected to 20 min ischemia/45 min reperfusion up to a concentration of 100 nM. The graph shows recovery of LVDP during reperfusion as a percent of paced baseline. The inset shows the percent recovery of the hearts at 45 min reperfusion for the rates of relaxation, contraction and coronary flow. There were no differences compared to control hearts not exposed to MRS5401.



[Figure ID: F5]
Figure 5 

The monomeric analogue, MRS5233, was effective at increasing functional recovery of isolated mouse hearts after 20 min ischemia/45 min reperfusion compared to control at 1 (A) and 10 nM (B). 1 nM MRS5233 showed a trend in improving LVDP over control during reperfusion, while there was significant improvement with 10 nM MRS5233. The insets show that the recovery of rates of relaxation and contraction at 45 min reperfusion was significantly better than that of control hearts at both concentrations.



[Figure ID: F6]
Figure 6 

The GLiDe conjugate, MRS5246, was effective at increasing functional recovery of isolated mouse hearts after 20 min ischemia/45 min reperfusion compared to control at 0.1 (A), 1 (B), and 10 nM (C). Both 1 and 10 nM concentrations of MRS5246 evoked significant improvement of LVDP over control during reperfusion. The insets show that there was also significantly better recovery of rates of relaxation and contraction at 45 min reperfusion than that of control hearts at both concentrations.



[Figure ID: F7]
Figure 7 

Hearts from A3KO mice exposed to 10 nM MRS5246 did not show improved functional recovery after I/R compared to control indicating the effects of MRS5246 were mA3AR-specific.



Tables
[TableWrap ID: T1] Table 1 

Binding affinities calculated from radiologand ([125I]I-AB-MECA) binding experiments.


Agonist mA3AR,
Ki or Kiapp (nM)
mA1AR,
Ki or Kiapp (nM)
Selectivity (A1 /A3)
MRS5246 0.04 ± 0.002 14.35 ± 4.35 359-fold

MRS5233 3.94 ± 0.31 665 ± 70 169-fold

98.5-fold increase
(MRS5233/MRS5246)
46.3-fold increase
(MRS5233/MRS5246)


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