Document Detail


Poly(amidoamine) conjugates containing doxorubicin bound via an acid-sensitive linker.
MedLine Citation:
PMID:  19016501     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Poly(amidoamine)s with amino pendant groups were prepared by hydrogen-transfer polyaddition of primary and secondary amines to bis-acrylamines. Dansyl cadaverine (DC) doxorubicin (Dox) were bound to the polymers via a cis-aconityl spacer to give conjugates containing 3 microg of DC per mg of polymer and 28 to 35 microg of Dox per mg of polymer. Release of DC and Dox at physiological and acidic pH varied from 0 to 35% over 48 h and was pH dependent. Although the ISA1Dox conjugate (IC(50) = 6 microg Dox x mL(-1)) presented similar toxicity as the parent polymer without Dox, ISA23Dox showed increased toxicity (IC(50) = 10 microg Dox x mL(-1)). These results suggest that ISA23Dox is able to release biologically active Dox in vitro and that this conjugate might be suitable for further development.
Authors:
Nathalie Lavignac; Johanna L Nicholls; Paolo Ferruti; Ruth Duncan
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Macromolecular bioscience     Volume:  9     ISSN:  1616-5195     ISO Abbreviation:  Macromol Biosci     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101135941     Medline TA:  Macromol Biosci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  480-7     Citation Subset:  IM    
Affiliation:
Centre for Polymer Therapeutics, Welsh School of Pharmacy, Redwood Building, Cardiff University, Cardiff, UK. n.lavignac@kent.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibiotics, Antineoplastic / chemistry*,  pharmacology
Biocompatible Materials / chemistry,  pharmacology
Cadaverine / analogs & derivatives,  chemistry
Cell Line, Tumor / drug effects
Doxorubicin / chemistry*,  pharmacology
Drug Carriers / chemistry*
Drug Screening Assays, Antitumor
Humans
Hydrogen-Ion Concentration
Materials Testing
Mice
Molecular Structure
Neoplasms / drug therapy
Polymers / chemistry*
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Biocompatible Materials; 0/Drug Carriers; 0/Polymers; 10121-91-2/monodansylcadaverine; 23214-92-8/Doxorubicin; 462-94-2/Cadaverine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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