Document Detail

Polyadenylation of the Xenopus beta 1 globin mRNA at a downstream minor site in the absence of the major site and utilization of an AAUACA polyadenylation signal.
MedLine Citation:
PMID:  2862026     Owner:  NLM     Status:  MEDLINE    
We show that adult reticulocytes of Xenopus laevis produce two forms of the beta 1 globin mRNA that differ in their site of polyadenylation. The minor site of polyadenylation is located 46 nucleotides downstream of the major site and is used in approximately 1% of mRNA molecules. A fusion gene was constructed containing the promoter from the thymidine kinase gene of herpes simplex virus fused to the protein coding, 3'-noncoding and 3'-flanking sequences of the X. laevis beta 1 globin gene. When injected into the nuclei of Xenopus oocytes, transcripts of this fusion gene were accurately and efficiently spliced and polyadenylated. The proportion of fusion gene transcripts terminating at the major and minor polyadenylation sites after injection into oocytes was approximately similar to that found in reticulocytes. When the AATAAA sequence element upstream from the major site was deleted, the minor site was used with a high (greater than 90%) efficiency. Therefore, by comparing the ratio of polyadenylation at the major and minor sites, it is possible to determine the effect of sequence alterations at the major site. In a construct where the AATAAA polyadenylation signal was changed to AATACA a high proportion (35%) of transcripts continued to be polyadenylated at the major site. This suggests a surprisingly high degree of flexibility in the precise polyadenylation signal.
P J Mason; M B Jones; J A Elkington; J G Williams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The EMBO journal     Volume:  4     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-08-30     Completed Date:  1985-08-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  205-11     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Globins / genetics*
Oocytes / metabolism
Poly A / genetics*,  metabolism
RNA Processing, Post-Transcriptional
RNA, Messenger / genetics*,  metabolism
Reticulocytes / metabolism
Transcription, Genetic
Xenopus laevis
Reg. No./Substance:
0/RNA, Messenger; 24937-83-5/Poly A; 9004-22-2/Globins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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