Document Detail


Poly(D,L-lactic acid)-block-(ligand-tethered poly(ethylene glycol)) copolymers as surface additives for promoting chondrocyte attachment and growth.
MedLine Citation:
PMID:  16130143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The poly(D,L-lactic acid)-block-(ligand-tethered poly(ethylene glycol)) copolymer was explored to engineer poly(D,L-lactic acid) (PLA) material to promote chondrocyte attachment and growth. The poly(D,L-lactic acid)-block-poly(ethylene glycol) copolymer (PLE) was synthesized by a coupling reaction between PLA and poly(ethylene glycol) (PEG) (M(n) 1000, 2000, and 4000 respectively), with the use of 4,4'-methylenediphenyl diisocyanate (MDI). Then the PLE was activated by methyl sulfonyl chloride and the amino acids or arginine-glycine-aspartic acid tripeptide (RGD) was attached, which was verified by the ninhydrin-UV method. The modified PLA films were simply prepared by blending PLA with PLE derivatives. ATR-FTIR, XPS, contact angle, and AFM results clearly showed that the PEG chain stably enriched on the surface of PLE-modified PLA films. The chondrocyte cytocompatibility test showed the modified PLA films could significantly improve chondrocyte attachment and proliferation.
Authors:
Guanhua Yu; Jian Ji; Huiguang Zhu; Jiacong Shen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  76     ISSN:  1552-4973     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-19     Completed Date:  2006-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  United States    
Other Details:
Languages:  eng     Pagination:  64-75     Citation Subset:  IM    
Affiliation:
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion / drug effects*
Cell Division / drug effects
Chondrocytes / cytology,  drug effects*,  ultrastructure
Microscopy, Confocal
Microscopy, Electron, Scanning
Polyesters / chemistry*
Polyethylene Glycols / chemistry*
Spectroscopy, Fourier Transform Infrared
Chemical
Reg. No./Substance:
0/Polyesters; 0/Polyethylene Glycols; 26969-66-4/poly(lactide)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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