Document Detail


Poly(ADP-ribose) polymerase 1 participates in the phase entrainment of circadian clocks to feeding.
MedLine Citation:
PMID:  20832105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Circadian clocks in peripheral organs are tightly coupled to cellular metabolism and are readily entrained by feeding-fasting cycles. However, the molecular mechanisms involved are largely unknown. Here we show that in liver the activity of PARP-1, an NAD(+)-dependent ADP-ribosyltransferase, oscillates in a daily manner and is regulated by feeding. We provide biochemical evidence that PARP-1 binds and poly(ADP-ribosyl)ates CLOCK at the beginning of the light phase. The loss of PARP-1 enhances the binding of CLOCK-BMAL1 to DNA and leads to a phase-shift of the interaction of CLOCK-BMAL1 with PER and CRY repressor proteins. As a consequence, CLOCK-BMAL1-dependent gene expression is altered in PARP-1-deficient mice, in particular in response to changes in feeding times. Our results show that Parp-1 knockout mice exhibit impaired food entrainment of peripheral circadian clocks and support a role for PARP-1 in connecting feeding with the mammalian timing system.
Authors:
Gad Asher; Hans Reinke; Matthias Altmeyer; Maria Gutierrez-Arcelus; Michael O Hottiger; Ueli Schibler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-09
Journal Detail:
Title:  Cell     Volume:  142     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2010-10-18     Revised Date:  2010-10-28    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  943-53     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland. gad.asher@unige.ch
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Clocks*
Circadian Rhythm*
Circadian Rhythm Signaling Peptides and Proteins / metabolism
Feeding Behavior*
Liver / metabolism
Mice
Mice, Knockout
Poly(ADP-ribose) Polymerases / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Circadian Rhythm Signaling Peptides and Proteins; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 2.4.2.30/poly(ADP-ribose)polymerase-1, mouse
Comments/Corrections
Comment In:
Cell. 2010 Sep 17;142(6):841-3   [PMID:  20850006 ]
Nat Rev Mol Cell Biol. 2010 Nov;11(11):754-5   [PMID:  20944668 ]

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