Document Detail


Poly(A) polymerase-based poly(a) length assay.
MedLine Citation:
PMID:  24590776     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
mRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.
Authors:
Deepak P Patil; Baskar Bakthavachalu; Daniel R Schoenberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1125     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2014  
Date Detail:
Created Date:  2014-03-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13-23     Citation Subset:  IM    
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