Document Detail


Poly-β-hydroxybutyrate (PHB) accumulating Bacillus spp. improve the survival, growth and robustness of Penaeus monodon () postlarvae.
MedLine Citation:
PMID:  25213234     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Low larval survival resulting from suboptimal culture conditions and luminous vibriosis poses a major problem for the larviculture of penaeid shrimp. In this study, a poly-β-hydroxybutyrate (PHB) accumulating mixed bacterial culture (mBC; 48.5% PHB on cell dry weight) and two PHB accumulating bacterial isolates, Bacillus sp. JL47 (54.7% PHB on cell dry weight) and Bacillus sp. JL1 (45.5% PHB on cell dry weight), were obtained from a Philippine shrimp culture pond and investigated for their capacity to improve growth, survival and robustness of Penaeus monodon postlarvae (PL). Shrimp PL1 and shrimp PL30 were provided with the PHB containing bacterial cultures in the feed for 30 days followed by, respectively, a challenge with pathogenic Vibrio campbellii and exposure to a lethal dose of ammonia. Prior to the pathogenic challenge or ammonia stress, growth and survival were higher for shrimp receiving the PHB accumulating bacteria as compared to shrimp receiving diets without bacterial additions. After exposure to the pathogenic challenge the shrimp fed PHB accumulating bacteria showed a higher survival as compared to non-treated shrimp, suggesting an increase in robustness for the shrimp. Similar effects were observed when shrimp PL30 were provided with the PHB accumulating bacterial cultures during a challenge with pathogenic V. campbellii through the water. The survival of shrimp exposed to lethal ammonia stress showed no significant difference between PHB accumulating bacteria-fed shrimp and non-PHB treated shrimp. The data illustrate that bacilli capable of accumulating PHB can provide beneficial effects to P. monodon post-larvae during culture in terms of growth performance, survival and resistance against pathogenic infection and ammonia stress. Further investigations are required to verify the PHB effect of the bacterial cultures on the shrimp.
Authors:
Joseph Leopoldo Q Laranja; Gladys L Ludevese-Pascual; Edgar C Amar; Patrick Sorgeloos; Peter Bossier; Peter De Schryver
Related Documents :
8933754 - Hyperthermia and hypoxia increase tolerance of retinal ganglion cells to anoxia and exc...
3624114 - Influence of stored aqueous ceftriaxone solutions on colony formation by neutrophil and...
1624124 - Isolation and properties of a low molecular mass endoglucanase from trichoderma reesei.
825804 - Paper point transfer of minimal numbers of microorganisms from a prepared canal in vitro.
2993634 - Paraquat toxicity in vitro. i. pulmonary alveolar macrophages.
16336214 - Cell binding, internalization and cytotoxic activity of human granzyme b expressed in t...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-20
Journal Detail:
Title:  Veterinary microbiology     Volume:  -     ISSN:  1873-2542     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-9-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier B.V. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Identification of Coxiella burnetii genotypes in Croatia using multi-locus VNTR analysis.
Next Document:  Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.