Document Detail

Poly-beta-hydroxybutyrate production by lactic acid bacteria.
MedLine Citation:
PMID:  9503624     Owner:  NLM     Status:  MEDLINE    
Poly-beta-hydroxybutyrate was determined in lactic acid bacteria belonging to the genera Lactobacillus, Lactococcus, Pediococcus and Streptococcus. Lactobacilli were grown in MRS broth, the others were grown in Elliker broth medium. Cell biomass was obtained by centrifugation. The cell walls were lysed with sodium hypochlorite. Poly-beta-hydroxybutyrate was extracted using chloroform in a Soxhlet system. Then it was converted to crotonic acid using sulfuric acid and the amount of crotonic acid was measured spectrophotometrically. The yield of poly-beta-hydroxybutyrate (% of cell dry weight) of Lactobacillus species was 6.6-35.8%. The values for Lactococcus, Pediococcus and Streptococcus species were 9.0-20.9, 1.1-8.0 and 6.8-17.2, respectively. It was observed that one of the Lactobacillus species did not produce poly-beta-hydroxybutyrate. Generally, Lactobacillus species produced more poly-beta-hydroxybutyrate than the other tested bacteria and no significant correlation was observed between poly-beta-hydroxybutyrate production and cell density of the cultures.
B Aslim; F Calişkan; Y Beyatli; U Gündüz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  159     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-26     Completed Date:  1998-03-26     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  293-7     Citation Subset:  IM    
Gazi University, Faculty of Arts and Science, Department of Biology, Ankara, Turkey.
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MeSH Terms
Hydroxybutyrates / metabolism*
Lactobacillus / metabolism*
Lactococcus / metabolism*
Pediococcus / metabolism*
Polyesters / metabolism*
Streptococcus / metabolism*
Reg. No./Substance:
0/Hydroxybutyrates; 0/Polyesters; 26063-00-3/poly-beta-hydroxybutyrate

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