| Poly-N-acetylglucosamine and poly(glycerol phosphate) teichoic acid identification from staphylococcal biofilm extracts using excitation sculptured TOCSY NMR. | |
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MedLine Citation:
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PMID: 18213410 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired (1)H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6)-N-acetylglucosamine (PNAG) and poly(glycerol phosphate) teichoic acid (TA) carbohydrate polymer components in crude biofilm extracts from Staphylococcus epidermidis without the need for further sample purification and component separation. Biofilms are implicated in the barriers for resistance of microbes toward antibiotics and immune responses, therefore efficient rapid detection and quantification of key components are important to assist in the design of a clinical infection response. |
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Authors:
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Jane L Wagstaff; Irina Sadovskaya; Evgeny Vinogradov; Saïd Jabbouri; Mark J Howard |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-23 |
Journal Detail:
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Title: Molecular bioSystems Volume: 4 ISSN: 1742-206X ISO Abbreviation: Mol Biosyst Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-23 Completed Date: 2008-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101251620 Medline TA: Mol Biosyst Country: England |
Other Details:
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Languages: eng Pagination: 170-4 Citation Subset: IM |
Affiliation:
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Protein Science Group, Department of Biosciences, University of Kent, Canterbury, Kent, UKCT2 7NJ. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biofilms* Magnetic Resonance Spectroscopy Staphylococcus / chemistry*, physiology* Teichoic Acids / chemistry* beta-Glucans / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Teichoic Acids; 0/beta-Glucans; 0/poly-N-acetyl-1-6-glucosamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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