Document Detail


Poly-N-acetylglucosamine and poly(glycerol phosphate) teichoic acid identification from staphylococcal biofilm extracts using excitation sculptured TOCSY NMR.
MedLine Citation:
PMID:  18213410     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired (1)H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6)-N-acetylglucosamine (PNAG) and poly(glycerol phosphate) teichoic acid (TA) carbohydrate polymer components in crude biofilm extracts from Staphylococcus epidermidis without the need for further sample purification and component separation. Biofilms are implicated in the barriers for resistance of microbes toward antibiotics and immune responses, therefore efficient rapid detection and quantification of key components are important to assist in the design of a clinical infection response.
Authors:
Jane L Wagstaff; Irina Sadovskaya; Evgeny Vinogradov; Saïd Jabbouri; Mark J Howard
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-23
Journal Detail:
Title:  Molecular bioSystems     Volume:  4     ISSN:  1742-206X     ISO Abbreviation:  Mol Biosyst     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-23     Completed Date:  2008-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101251620     Medline TA:  Mol Biosyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  170-4     Citation Subset:  IM    
Affiliation:
Protein Science Group, Department of Biosciences, University of Kent, Canterbury, Kent, UKCT2 7NJ.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biofilms*
Magnetic Resonance Spectroscopy
Staphylococcus / chemistry*,  physiology*
Teichoic Acids / chemistry*
beta-Glucans / chemistry*
Chemical
Reg. No./Substance:
0/Teichoic Acids; 0/beta-Glucans; 0/poly-N-acetyl-1-6-glucosamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Altered acetate metabolism and biomass production in several Escherichia coli mutants lacking rpoS-d...
Next Document:  Fluid bridges confined between chemically nanopatterned solid substrates.