Document Detail


Poly-3-hydroxybutyrate metabolism in the type II methanotroph Methylocystis parvus OBBP.
MedLine Citation:
PMID:  21724874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differences in carbon assimilation pathways and reducing power requirements among organisms are likely to affect the role of the storage polymer poly-3-hydroxybutyrate (PHB). Previous researchers have demonstrated that PHB functions as a sole growth substrate in aerobic cultures enriched on acetate during periods of carbon deficiency, but it is uncertain how C(1) metabolism affects the role of PHB. In the present study, the type II methanotroph Methylocystis parvus OBBP did not replicate using stored PHB in the absence of methane, even when all other nutrients were provided in excess. When PHB-rich cultures of M. parvus OBBP were deprived of carbon and nitrogen for 48 h, they did not utilize significant amounts of stored PHB, and neither cell concentrations nor concentrations of total suspended solids changed significantly. When methane and nitrogen both were present, PHB and methane were consumed simultaneously. Cells with PHB had significantly higher specific growth rates than cells lacking PHB. The addition of formate (a source of reducing power) to PHB-rich cells delayed PHB consumption, but the addition of glyoxylate (a source of C(2) units) did not. This and results from other researchers suggest that methanotrophic PHB metabolism is linked to the supply of reducing power as opposed to the supply of C(2) units for synthesis.
Authors:
Allison J Pieja; Eric R Sundstrom; Craig S Criddle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-07-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-25     Completed Date:  2011-12-13     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6012-9     Citation Subset:  IM    
Affiliation:
Environmental, Engineering and Science, Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020, USA.
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MeSH Terms
Descriptor/Qualifier:
Carbon / metabolism
Formates / metabolism
Glyoxylates / metabolism
Hydroxybutyrates / metabolism*
Methane / metabolism
Methylocystaceae / metabolism*
Nitrogen / metabolism
Polyesters / metabolism*
Chemical
Reg. No./Substance:
0/Formates; 0/Glyoxylates; 0/Hydroxybutyrates; 0/Polyesters; 26063-00-3/poly-beta-hydroxybutyrate; 64-18-6/formic acid; 74-82-8/Methane; 7440-44-0/Carbon; 7727-37-9/Nitrogen; JQ39C92HH6/glyoxylic acid
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