| Poly-2'-deoxy-2'-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acid. | |
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MedLine Citation:
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PMID: 896478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The polymerization of 2'deoxy-2'-fluoro-cytidine-diphosphate (dCflDP) by polynucleotide phosphorylase is barely detectable in the presence of Mg++ under usual experimental conditions for polymerization of nucleoside diphosphates. High concentrations of enzyme have to be used to accomplish the synthesis. Mn++ is a better activator than Mg++ for the reaction. cCflDP inhibits the polymerization of CDP and has a Km=8.8X10-3M, six times higher than CDP.- The polymer, poly (dCfl), ressembles in many respects poly(C), but not poly(dC): the acid selfstructure forms at similar pK's; interaction with poly(I) yields a 1:1 complex the CD spectrum of which is similar to that of poly(I).poly(C). Finally, the Tm's of poly(I).poly(dCfl) are comparable to those of poly(I).poly(C). |
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Authors:
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W Guschlbauer; M Blandin; J L Drocourt; M N Thang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids research Volume: 4 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1977 Jun |
Date Detail:
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Created Date: 1977-10-14 Completed Date: 1977-10-14 Revised Date: 2010-09-03 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1933-43 Citation Subset: IM |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Circular Dichroism Cytosine Nucleotides / metabolism* Deoxycytidine Monophosphate / metabolism* Hot Temperature Kinetics Magnesium / pharmacology Manganese / pharmacology Nucleic Acid Denaturation Poly C / analogs & derivatives, biosynthesis* Poly I Poly I-C Polydeoxyribonucleotides / biosynthesis* Polyribonucleotide Nucleotidyltransferase / metabolism Polyribonucleotides / biosynthesis* Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Cytosine Nucleotides; 0/Polydeoxyribonucleotides; 0/Polyribonucleotides; 1032-65-1/Deoxycytidine Monophosphate; 24939-03-5/Poly I-C; 25249-22-3/Poly I; 30811-80-4/Poly C; 7439-95-4/Magnesium; 7439-96-5/Manganese; EC 2.7.7.8/Polyribonucleotide Nucleotidyltransferase |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Month: 6 Year: 1977 Volume: 4 Issue: 6 First Page: 1933 Last Page: 1943 ID: 342532 PubMed Id: 896478 |
| Poly-2'-deoxy-2'-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acid. | |
| W Guschlbauer | |
| M Blandin | |
| J L Drocourt | |
| M N Thang | |
Article Categories:
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