Document Detail

Poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site I.
MedLine Citation:
PMID:  2838906     Owner:  NLM     Status:  MEDLINE    
The mouse-adapted strain of poliovirus type 2 (Lansing) induces fatal poliomyelitis in mice after intracerebral inoculation, whereas mice inoculated with poliovirus type 1 (Mahoney) show no signs of disease. Previous work indicated that the adaptation to mouse virulence is associated with the viral capsid proteins and that mutations in neutralization antigenic site I of poliovirus reduce neurovirulence of the Lansing strain in mice. The role of antigenic site I in mouse neurovirulence was further explored by constructing an antigenic hybrid virus. Six amino acids in antigenic site I of the Mahoney strain were replaced with a sequence specific for the Lansing strain by using a mutagenesis cartridge. The hybrid virus was neutralized by polyclonal antisera elicited by the type 1 and type 2 strains of poliovirus and by neutralizing monoclonal antibodies directed against antigenic site I of type 2 virus. The hybrid virus induced paralytic disease in mice, an observation demonstrating that a short sequence of amino acids in antigenic site I is an important determinant of poliovirus host range. Antigenic site I may be involved in attachment of poliovirus to cells of the mouse central nervous system.
M G Murray; J Bradley; X F Yang; E Wimmer; E G Moss; V R Racaniello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  241     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-03     Completed Date:  1988-08-03     Revised Date:  2014-02-03    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  213-5     Citation Subset:  IM    
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MeSH Terms
Antibodies, Viral / immunology
Antigens, Viral / physiology*
Capsid / physiology
DNA Mutational Analysis
Nervous System Diseases / microbiology
Neutralization Tests
Poliovirus / growth & development,  immunology,  pathogenicity*
Virus Replication
Grant Support
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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