Document Detail

Polarization and symmetry of electronic transitions in long fluorescence lifetime triangulenium dyes.
MedLine Citation:
PMID:  23391292     Owner:  NLM     Status:  MEDLINE    
To fully exploit the capabilities of fluorescence probes in modern experiments, where advanced instrumentation is used to probe complex environments, other photophysical properties than emission color and emission intensity are monitored. Each dye property can be addressed individually as well as collectively to provide in-depth information unavailable from the standard intensity measurements. Dyes with long emission lifetimes and strongly polarized transitions enable the monitoring of lifetime changes as well as emission polarization (anisotropy). Thus experiments can be designed to follow slow dynamics. The UV and visible electronic transitions of a series of red-emitting dyes based on the triangulenium motif are investigated. We resolve overlapping features in the spectra and assign the orientation of the transition moments to the molecular axes. The result is the complete Jablonski diagram for the UV and visible spectral region. The symmetries of the studied dyes are shown to have a large influence on the optical response, and they are clearly separated into two groups of symmetry by their photophysical properties. The C(2v) symmetric dyes, azadioxatriangulenium (ADOTA(+)) and diazaoxatriangulenium (DAOTA(+)), have high emission anisotropies, fluorescence lifetimes around 20 ns, and fluorescence quantum yields of ∼50%. The trioxatriangulenium (TOTA(+)) and triazatriangulenium (TATA(+)) dyes-nominally of D(3h) symmetry-have fluorescence lifetimes around 10 ns lifetimes and fluorescence quantum yields of 10-15%. However, the D(3h) symmetry is shown to be lowered to a point group, where the axes transform uniquely such that the degeneracy of the E' states is lifted.
Erling Thyrhaug; Thomas Just Sørensen; Ignacy Gryczynski; Zygmunt Gryczynski; Bo W Laursen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-06
Journal Detail:
Title:  The journal of physical chemistry. A     Volume:  117     ISSN:  1520-5215     ISO Abbreviation:  J Phys Chem A     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-14     Completed Date:  2013-08-27     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  9890903     Medline TA:  J Phys Chem A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2160-8     Citation Subset:  IM    
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MeSH Terms
Fluorescent Dyes / analysis,  chemical synthesis*
Pyrenes / analysis,  chemical synthesis*
Quantum Theory
Grant Support
Reg. No./Substance:
0/Fluorescent Dyes; 0/Pyrenes; 0/trioxatriangulenium

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