Document Detail


Polarization of the Golgi apparatus and the microtubule-organizing center within cloned natural killer cells bound to their targets.
MedLine Citation:
PMID:  6359165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In cell couples formed between a cloned murine natural killer (NK) cell and either of two target cells, we have obtained evidence by immunofluorescence observations for a rapid coordinate repositioning of two organelles, the microtubule organizing center and the perinuclear Golgi apparatus, inside the NK cell to face the region of contact with the target cell. With microtubule-disrupting drugs, which also cause a dispersion of the Golgi apparatus throughout the periphery of the NK cell, it was found that target cell lysis is reversibly inhibited. It is proposed that the coordinate repositioning of the two organelles serves the function of directing secretory vesicles derived from the Golgi apparatus to the bound target cells, the secretory vesicles containing components that induce target cell lysis.
Authors:
A Kupfer; G Dennert; S J Singer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  80     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7224-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cells, Cultured
Clone Cells
Cytotoxicity, Immunologic
Fluorescent Antibody Technique
Golgi Apparatus / ultrastructure*
Killer Cells, Natural / immunology,  ultrastructure*
Lymphoma / immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microtubules / ultrastructure*
Plasmacytoma / immunology
Grant Support
ID/Acronym/Agency:
AI-06659/AI/NIAID NIH HHS; CA-15581/CA/NCI NIH HHS; GM-15971/GM/NIGMS NIH HHS
Comments/Corrections

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