Document Detail


Polar glycerolipids of Chlamydomonas moewusii.
MedLine Citation:
PMID:  10680181     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fatty acid and polar lipid compositions of the unicellular green alga Chlamydomonas moewusii were characterized. Since this organism is an important plant model for phospholipid-based signal transduction, interest was focused on the lipids phosphatidic acid, phosphatidylinositolphosphate and phosphatidylinositolbisphosphate. A phosphatidylinositol:phosphatidylinositolphosphate: phosphatidylinositolbisphosphate ratio of 100:1.7:1.3 was found. The polyphosphoinositides accounted for 0.8 mol% of the total phospholipids and their fatty acid compositions were similar to that of phosphatidylinositol except for the enrichment of linolenic acid in phosphatidylinositol phosphate. Phosphatidic acid accounted for 0.67 mol% of the phospholipids. Major structural glycerolipids were monogalactosyldiacylglycerol (35 mol%), digalactosyldiacylglycerol (15 mol%), sulfoquinovosyldiacylglycerol (10 mol%), diacylglyceryltrimethylhomoserine (16 mol%), phosphatidylglycerol (9 mol%), phosphatidylethanolamine (8 mol%) and phosphatidylinositol (6 mol%). Relative changes in the total fatty acid compositions found during growth on nutrient-limited medium reflected mainly alterations in the compositions of the chloroplast lipids phosphatidylglycerol and monogalactosyldiacylglycerol. [32P]Pi-incorporation studies revealed that it took 6 days before the amount of label in the major phospholipids was proportional to their abundance.
Authors:
S A Arisz; J A van Himbergen; A Musgrave; H van den Ende; T Munnik
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytochemistry     Volume:  53     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-03-15     Completed Date:  2000-03-15     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  265-70     Citation Subset:  IM    
Affiliation:
Institute for Molecular Cell Biology, University of Amsterdam, The Netherlands. arisz@bio.uva.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Chlamydomonas / chemistry,  metabolism*
Glycerides / biosynthesis*,  chemistry,  isolation & purification
Kinetics
Phosphates / metabolism
Phospholipids / biosynthesis*,  chemistry,  isolation & purification
Phosphorus Radioisotopes
Chemical
Reg. No./Substance:
0/Glycerides; 0/Phosphates; 0/Phospholipids; 0/Phosphorus Radioisotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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