Document Detail


Polar body diagnosis of common aneuploidies by FISH.
MedLine Citation:
PMID:  8688589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The purpose of this work was to investigate the reliability and accuracy of polar body analysis for preimplantation diagnosis of common aneuploidies in IVF patients of advanced maternal age. DESIGN: We have previously introduced polar body analysis as an approach for nondestractive evaluation of the genotype of human oocytes. The method has recently been applied in a clinical trial involving 45 infertile patients, demonstrating the feasibility of preconception diagnosis of common aneuploidies by fluorescent in situ hybridization (FISH). The present paper describes the experience of polar body diagnosis in 135 IVF patients (161 cycles) of advanced maternal age. RESULTS: FISH results of the first and/or second polar bodies were available in 648 (72.4%) of 895 biopsied oocytes subjected to FISH analysis. Of 648 oocytes with FISH results, 208 demonstrated chromosomal abnormalities. Of 440 oocytes predicted to be free from monosomy or trisomy of chromosomes X, 18, and/or 13/21, 314 were normally fertilized, cleaved, and transferred in 122 treatment cycles, resulting in 6 healthy deliveries and 12 ongoing pregnancies following confirmation of the polar body diagnosis by CVS or amniocentesis. CONCLUSIONS: The method may be useful for detection of oocytes with common chromosomal trisomies in IVF patients of advanced maternal age.
Authors:
Y Verlinsky; J Cieslak; M Freidine; V Ivakhnenko; G Wolf; L Kovalinskaya; M White; A Lifchez; B Kaplan; J Moise; J Valle; N Ginsberg; C Strom; A Kuliev
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  13     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-08-26     Completed Date:  1996-08-26     Revised Date:  2008-03-18    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  157-62     Citation Subset:  IM    
Affiliation:
Reproductive Genetics Institute, Illinois Masonic Medical Center, Chicago 60657, USA.
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MeSH Terms
Descriptor/Qualifier:
Abortion, Spontaneous / genetics
Adult
Aneuploidy*
Chromosome Aberrations / diagnosis*,  embryology,  genetics,  prevention & control
Chromosome Disorders
Feasibility Studies
Female
Fertilization in Vitro / methods*
Fetal Diseases / prevention & control*
Humans
In Situ Hybridization, Fluorescence / methods*
Infant, Newborn
Karyotyping
Maternal Age
Meiosis / genetics*
Nondisjunction, Genetic
Oocytes / ultrastructure
Pregnancy
Pregnancy Outcome
Pregnancy Rate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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