Document Detail


Polaprezinc protects human colon cells from oxidative injury induced by hydrogen peroxide: relevant to cytoprotective heat shock proteins.
MedLine Citation:
PMID:  17036391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the effect of polaprezinc on cellular damage induced by hydrogen peroxide (H(2)O(2)) in human colon CaCo2 cells.
METHODS: CaCo2 cells were treated with polaprezinc (10-100 micromol/L) for 6 h. After polaprezinc treatment, the cells were incubated with H(2)O(2) (20 micromol/L) for 1 h. Cell viability was measured by MTT assay. Western blot analysis for heat shock protein (HSP) 27 and HSP72 in the cells was performed. Moreover, cells were pretreated with quercetin (200 micromol/L), an inhibitor of HSP synthesis, 2 h before polaprezinc treatment, and cell viability and the expression of HSP27 and 72 were assessed in these cells.
RESULTS: Polaprezinc significantly protected CaCo2 cells from cell damage induced by H(2)O(2), and up-regulated the expressions of HSP27 and HSP72 in the cells (10, 30 and 100 micromol/L of polaprezinc; 35.0% +/- 7.7%, 58.3% +/- 14.6% and 64.2% +/- 8.2%, respectively. P < 0.01 versus polaprezinc-nontreated cells; 6.0% +/- 4.4%). Quercetin inhibited the up-regulation of HSP27 and HSP72 by polaprezinc and diminished the protective effect of polaprezinc against H(2)O(2)-caused injury in the cells.
CONCLUSION: Polaprezinc is a useful therapeutic agent for treatment of colitis and its effects depend on the function of cytoprotective HSP in colon.
Authors:
Tatsuya Ohkawara; Jun Nishihira; Rika Nagashima; Hiroshi Takeda; Masahiro Asaka
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  12     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-12     Completed Date:  2006-12-05     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  6178-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anti-Ulcer Agents / pharmacology*
Caco-2 Cells
Carnosine / analogs & derivatives*,  pharmacology
Colon / drug effects*
Gene Expression
HSP27 Heat-Shock Proteins
HSP72 Heat-Shock Proteins / antagonists & inhibitors,  drug effects
Heat-Shock Proteins / antagonists & inhibitors,  drug effects*
Humans
Hydrogen Peroxide / pharmacology
Neoplasm Proteins / antagonists & inhibitors,  drug effects
Organometallic Compounds / pharmacology*
Oxidative Stress / drug effects*
Quercetin / pharmacology
Up-Regulation
Zinc Compounds / pharmacology
Chemical
Reg. No./Substance:
0/Anti-Ulcer Agents; 0/HSP27 Heat-Shock Proteins; 0/HSP72 Heat-Shock Proteins; 0/HSPB1 protein, human; 0/Heat-Shock Proteins; 0/Neoplasm Proteins; 0/Organometallic Compounds; 0/Zinc Compounds; 107667-60-7/polaprezinc; 8HO6PVN24W/Carnosine; 9IKM0I5T1E/Quercetin; BBX060AN9V/Hydrogen Peroxide
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