Document Detail


Point mutations in EBV gH that abrogate or differentially affect B cell and epithelial cell fusion.
MedLine Citation:
PMID:  17307213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell fusion mediated by Epstein-Barr virus requires three conserved glycoproteins, gB and gHgL, but activation is cell type specific. B cell fusion requires interaction between MHC class II and a fourth virus glycoprotein, gp42, which complexes non-covalently with gHgL. Epithelial cell fusion requires interaction between gHgL and a novel epithelial cell coreceptor and is blocked by excess gp42. We show here that gp42 interacts directly with gH and that point mutations in the region of gH recognized by an antibody that differentially inhibits epithelial and B cell fusion significantly impact both the core fusion machinery and cell-specific events. Substitution of alanine for glycine at residue 594 completely abrogates fusion with either B cells or epithelial cells. Substitution of alanine for glutamic acid at residue 595 reduces fusion with epithelial cells, greatly enhances fusion with B cells and allows low levels of B cell fusion even in the absence of gL.
Authors:
Liguo Wu; Lindsey M Hutt-Fletcher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-02-20
Journal Detail:
Title:  Virology     Volume:  363     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-08     Completed Date:  2007-10-12     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  148-55     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acid Substitution
Animals
B-Lymphocytes / cytology*,  virology
Cell Fusion
Cell Line
Epithelial Cells / cytology*,  virology
Glycoproteins / chemistry,  genetics*,  metabolism*
Herpesvirus 4, Human / genetics*,  metabolism*
Molecular Sequence Data
Point Mutation / genetics*
Viral Proteins / chemistry,  genetics,  metabolism
Virus Internalization*
Grant Support
ID/Acronym/Agency:
AI20662/AI/NIAID NIH HHS; DE016669/DE/NIDCR NIH HHS; P20-RR018724/RR/NCRR NIH HHS; R01 AI020662/AI/NIAID NIH HHS; R01 AI020662-22/AI/NIAID NIH HHS; R01 DE016669-01/DE/NIDCR NIH HHS; R01 DE016669-02/DE/NIDCR NIH HHS; R01 DE016669-07/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/BZLF2 protein, Herpesvirus 4, Human; 0/Glycoproteins; 0/Viral Proteins
Comments/Corrections

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