Document Detail

Podoplanin, a novel marker of tumor-initiating cells in human squamous cell carcinoma A431.
MedLine Citation:
PMID:  18539139     Owner:  NLM     Status:  MEDLINE    
Squamous cell carcinoma (SCC) is a malignant tumor that shows morphologic and phenotypic similarities to normally differentiated squamous epithelium. Thus, it may be an ideal model for seeking a marker of tumor-initiating cells (TICs) based on their morphology. Using the human SCC cell line A431, we found that, as a paradigm of cancer stem cells: (1) podoplanin(+) cells generate both podoplanin(+) and podoplanin(-) cells; (2) podoplanin(-) cells rarely generate podoplanin(+) cells; (3) podoplanin(+) cells have higher colony formation efficiency and tumorigenicity than podoplanin(-) cells; (4) localization and morphology of podoplanin(+) cells in a xenografted tumor derived from podoplanin(+) cells are similar with those in human oral SCC tissue or normal epithelium. Furthermore, podoplanin(+) A431 cells share sonic hedgehog and CD44 expression with stem cells in normal squamous epithelium. Hence, we concluded that podoplanin is a novel marker to enrich TICs with stem-cell-like properties from SCC cell line A431.
Naho Atsumi; Genichiro Ishii; Motohiro Kojima; Masaru Sanada; Satoshi Fujii; Atsushi Ochiai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-06
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  373     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-01     Completed Date:  2008-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  36-41     Citation Subset:  IM    
Laboratory of Cancer Biology, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan.
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MeSH Terms
Antigens, CD44 / analysis
Carcinoma, Squamous Cell / diagnosis*
Cell Line, Tumor
Hedgehog Proteins / analysis
Membrane Glycoproteins / analysis*
Mice, SCID
Mouth Mucosa / chemistry
Neoplastic Stem Cells / chemistry*
Transplantation, Heterologous
Tumor Markers, Biological / analysis*
Reg. No./Substance:
0/Antigens, CD44; 0/Hedgehog Proteins; 0/Membrane Glycoproteins; 0/PDPN protein, human; 0/SHH protein, human; 0/Tumor Markers, Biological

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