Document Detail


Podoplanin expression in basal and myoepithelial cells: utility and potential pitfalls.
MedLine Citation:
PMID:  20042851     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Podoplanin, D2-40, is often used for highlighting lymphatics. However, it has been described in a variety of normal and neoplastic tissues. We evaluated the expression of D2-40 in breast, salivary gland, skin, and mucosa, all organs rich in myoepithelial cells and basal cells. This study assessed the utility of using D2-40 to highlight basal/myoepithelial cells and to identify potential pitfalls in misinterpretation of lymphatic invasion. Our results showed that myoepithelial cells in breast and salivary gland and basal cells in prostate consistently demonstrate D2-40 immunoreactivity, but typically less intensely than lymphatics. Cutaneous and mucosal-based basal cells also have D2-40 expression, but often in a patchy, focal manner. In addition, many salivary gland tumors and squamous cell carcinomas had strong D2-40 expression, sometimes making distinction of lymphatics versus tumor edge staining difficult. D2-40 is excellent for assessing lymphatic invasion in breast, prostate, and cervix as long as the pathologist is aware of the expression in myoepithelial cells/basal cells to avoid misinterpretation of ductal carcinoma in situ or normal prostate glands or tumor stroma retraction as tumor lymphatic invasion. Given the patchy positivity in basal cells of skin and mucosa and the reactivity in squamous cell carcinoma, D2-40 was not helpful in assessing for microinvasion of squamous cell carcinoma.
Authors:
William A Kanner; Mary T Galgano; Kristen A Atkins
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry     Volume:  18     ISSN:  1533-4058     ISO Abbreviation:  Appl. Immunohistochem. Mol. Morphol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-09-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888796     Medline TA:  Appl Immunohistochem Mol Morphol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  226-30     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Virginia Health System, Charlottesville, VA 22901, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Breast / metabolism,  pathology
Carcinoma, Squamous Cell / diagnosis,  metabolism,  pathology
Diagnostic Errors
Female
Humans
Immunohistochemistry
Lymphatic Metastasis / diagnosis*
Lymphatic Vessels / metabolism,  pathology
Male
Membrane Glycoproteins / immunology,  metabolism*
Mucous Membrane / metabolism,  pathology
Neoplasms / diagnosis*,  metabolism,  pathology*
Prostate / metabolism,  pathology
Salivary Gland Neoplasms / diagnosis,  metabolism,  pathology
Salivary Glands / metabolism,  pathology
Skin / metabolism,  pathology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Membrane Glycoproteins; 0/PDPN protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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