| Podoplanin expression in basal and myoepithelial cells: utility and potential pitfalls. | |
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MedLine Citation:
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PMID: 20042851 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Podoplanin, D2-40, is often used for highlighting lymphatics. However, it has been described in a variety of normal and neoplastic tissues. We evaluated the expression of D2-40 in breast, salivary gland, skin, and mucosa, all organs rich in myoepithelial cells and basal cells. This study assessed the utility of using D2-40 to highlight basal/myoepithelial cells and to identify potential pitfalls in misinterpretation of lymphatic invasion. Our results showed that myoepithelial cells in breast and salivary gland and basal cells in prostate consistently demonstrate D2-40 immunoreactivity, but typically less intensely than lymphatics. Cutaneous and mucosal-based basal cells also have D2-40 expression, but often in a patchy, focal manner. In addition, many salivary gland tumors and squamous cell carcinomas had strong D2-40 expression, sometimes making distinction of lymphatics versus tumor edge staining difficult. D2-40 is excellent for assessing lymphatic invasion in breast, prostate, and cervix as long as the pathologist is aware of the expression in myoepithelial cells/basal cells to avoid misinterpretation of ductal carcinoma in situ or normal prostate glands or tumor stroma retraction as tumor lymphatic invasion. Given the patchy positivity in basal cells of skin and mucosa and the reactivity in squamous cell carcinoma, D2-40 was not helpful in assessing for microinvasion of squamous cell carcinoma. |
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Authors:
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William A Kanner; Mary T Galgano; Kristen A Atkins |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry Volume: 18 ISSN: 1533-4058 ISO Abbreviation: Appl. Immunohistochem. Mol. Morphol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-23 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888796 Medline TA: Appl Immunohistochem Mol Morphol Country: United States |
Other Details:
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Languages: eng Pagination: 226-30 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Virginia Health System, Charlottesville, VA 22901, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal Breast / metabolism, pathology Carcinoma, Squamous Cell / diagnosis, metabolism, pathology Diagnostic Errors Female Humans Immunohistochemistry Lymphatic Metastasis / diagnosis* Lymphatic Vessels / metabolism, pathology Male Membrane Glycoproteins / immunology, metabolism* Mucous Membrane / metabolism, pathology Neoplasms / diagnosis*, metabolism, pathology* Prostate / metabolism, pathology Salivary Gland Neoplasms / diagnosis, metabolism, pathology Salivary Glands / metabolism, pathology Skin / metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Membrane Glycoproteins; 0/PDPN protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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