Document Detail

Podofilox-induced regression of Shope papillomas may be independent of host immunity.
MedLine Citation:
PMID:  8245513     Owner:  NLM     Status:  MEDLINE    
We tested the hypothesis that infiltrating leukocytes might contribute to papilloma destruction following podofilox treatment. New Zealand White (NZW) rabbits were inoculated with cottontail rabbit papillomavirus (CRPV) onto abraded areas of the dorsal skin. At 21 d after viral inoculation, 5.0% podofilox solution was applied to some papillomas, whereas others were used as controls. Three rabbits were sacrificed at each of three different periods after treatment initiation (1, 4, and 7 d). Four monoclonal antibodies (MoAbs), RG-16 (for B cells), L11/135 (specific for T cells), 2C4 (specific for class II antigen), and Ki67 (specific for proliferating cells), were used in an immunohistochemical study. All positive cells and total cells in the field were counted with an ocular grid. After 1 d of treatment, proliferation of papilloma cells was strongly suppressed in treated papillomas, but leukocytic infiltration was not altered. At 4 d and 7 d of treatment, there were substantial increases (about two to three times) in the numbers of B and T cells and class II-expressing leukocytes. The upper layers of the papillomas were highly necrotic and cell proliferation was absent in all layers. These data support the view that podofilox has a direct toxic effect on papilloma tissue. Leukocyte infiltration is not strongly associated with papilloma tissue and may not contribute to papilloma destruction.
M Okabayashi; M D Pickel; L R Budgeon; N M Cladel; J W Kreider
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  101     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-01-06     Completed Date:  1994-01-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  852-7     Citation Subset:  IM    
Department of Pathology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
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MeSH Terms
Antibodies, Monoclonal / immunology
B-Lymphocytes / cytology
Cell Transformation, Neoplastic
Cottontail rabbit papillomavirus*
Histocompatibility Antigens Class II / immunology
Ki-67 Antigen
Neoplasm Proteins / analysis
Nuclear Proteins / analysis
Papillomavirus Infections / drug therapy*,  immunology*
Podophyllotoxin / therapeutic use*
Skin / cytology,  immunology
T-Lymphocytes / cytology
Tumor Virus Infections / drug therapy*,  immunology*,  pathology
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Histocompatibility Antigens Class II; 0/Ki-67 Antigen; 0/Neoplasm Proteins; 0/Nuclear Proteins; 518-28-5/Podophyllotoxin

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