Document Detail

Podocytes in aminonucleoside glomerulonephritis investigated ultrastructurally with concanavalin A.
MedLine Citation:
PMID:  521617     Owner:  NLM     Status:  MEDLINE    
Peroxidase labeled concanavalin A (Con A) permits the detection of some saccharide determinants. This histochemical technique permits the visualization of cellular pathological modifications not observed with other methods. Its use in an ultrastructural study of nephritis induced by a single injection of aminonucleoside demonstrated the following in podocytes. The Con A positive endoplasmic reticulum (ER), essentially the rough ER, lost its normal linear and network appearance to take on a dot dash pattern. ER contents but not attached ribosomes and membranes were Con A positive. The dot dash pattern, due to a fragmentation of the ER, appeared prior to the onset of proteinuria and was attenuated before the disappearance of proteinuria. These changes of the ER were not observed in other proteinuric states. This suggests that aminonucleoside can damage the synthesis apparatus of podocytes, revealed by the Con A method.
R Bretton; M Rouchon; J Bariety
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  27     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-03-27     Completed Date:  1980-03-27     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1588-95     Citation Subset:  IM    
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MeSH Terms
Concanavalin A / diagnostic use*
Cytoplasm / ultrastructure
Endoplasmic Reticulum / analysis,  ultrastructure
Glomerulonephritis / chemically induced,  pathology*
Kidney Glomerulus / ultrastructure*
Puromycin Aminonucleoside
Vacuoles / ultrastructure
Reg. No./Substance:
11028-71-0/Concanavalin A; 58-60-6/Puromycin Aminonucleoside

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