Document Detail


Podocytes contribute to the formation of glomerular crescents.
MedLine Citation:
PMID:  18199804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cellular composition of crescents in glomerular disease is controversial. The role of podocytes in crescent formation has been especially difficult to study because podocytes typically lose their characteristic terminally differentiated phenotype under disease conditions, making them difficult to identify. We reasoned that the intermediate filament protein nestin, a marker of progenitor cells that has recently been identified in podocytes, may allow the investigation of podocyte involvement in glomerular crescents. In a series of 35 biopsies with crescentic glomerular disease, all showed nestin-positive cells in the crescents, ranging in number from occasional to approximately 50% of crescent cells. Other podocyte markers, such as podocin and WT1, failed to identify cells in crescents, and no contribution by endothelial or myogenic cells was noted. CD68-positive cells were observed in 80% of cases but were never as numerous as the nestin-positive cells. Nestin and CD68 were not coexpressed by the same cells, providing no evidence of trans-differentiation of podocytes into a macrophage phenotype. Keratin-positive cells were found in crescents in 51% of cases, but only as occasional cells. Up to one third of crescent cells were cycling in 48% of biopsies, and double immunostaining identified these cells as a mixture of nestin-positive cells and "null" cells (negative for nestin, CD68, and keratin). In addition to our observations in human disease, we also identified nestin-positive proliferating podocytes in the crescents of 2 mouse models of crescentic glomerulonephritis. We conclude that podocytes play a role in the formation of glomerular crescents.
Authors:
Paul S Thorner; Michael Ho; Vera Eremina; Yoshikazu Sado; Susan Quaggin
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Publication Detail:
Type:  Journal Article     Date:  2008-01-16
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  19     ISSN:  1533-3450     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-29     Completed Date:  2008-03-26     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  495-502     Citation Subset:  IM    
Affiliation:
Division of Pathology, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada. paul.thorner@sickkids.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Glomerular Basement Membrane Disease / metabolism,  pathology*
Cell Cycle / physiology
Humans
Immunophenotyping
Intermediate Filament Proteins / metabolism*
Ki-67 Antigen / metabolism
Mice
Nerve Tissue Proteins / metabolism*
Podocytes / metabolism,  physiology*
Chemical
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/Ki-67 Antigen; 0/Nerve Tissue Proteins; 0/nestin
Comments/Corrections

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