| Pneumocystis carinii f. sp. carinii synthesizes de novo four homologs of ubiquinone. | |
| | |
MedLine Citation:
|
PMID: 12095106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ubiquinone, coenzyme Q, plays a pivotal role in electron transport and is a target for chemotherapy against a number of eukaryotic infectious agents, including Pneumocystis carinii. Coenzyme Q10 was previously identified as the major ubiquinone homolog in P. carinii isolated and purified from rat lungs; CoQ9 was also present. In contrast, CoQ9 and CoQ8 (but not CoQ10) were detected in the lungs of uninfected rat controls. These observations suggested that the pathogen synthesizes CoQ10, and perhaps CoQ9 as well. In the present study, CoQ biosynthesis in P. carinii was examined in greater detail. Radiolabeled mevalonate, a precursor of the CoQ polyprenyl chain, was incorporated in vitro into P. carinii ubiquinones. Incorporation of radiolabeled mevalonate into P. carinii CoQ was not enhanced by treating cells with lovastatin, suggesting that the cells did not transport the drug, or that a lovastatin-insensitive pathway for de novo synthesis of isoprenoids may also function in this organism. Radiolabeled precursors of the ring moiety, including shikimic acid, p-hydroxybenzoic acid, and tyrosine were also incorporated into P. carinii CoQ. Unexpectedly, it was found that not only CoQ9 and CoQ10, but also CoQ7, and CoQ8, were metabolically radiolabeled by all the precursors tested, indicating that the organism synthesizes CoQ7, CoQ8, CoQ9, and CoQ10. Metabolic radiolabeling of ubiquinones in rat lung controls was not detected in experiments using either radioactive mevalonate or p-hydroxybenzoate. Thus the incorporations measured using purified P. carinii preparations were due to the enzymes of the organism. |
| | |
Authors:
|
D Sul; E S Kaneshiro |
Related Documents
:
|
11114786 - A retrospective review of the carcinogenicity of refractory ceramic fiber in two chroni... 559016 - Effect of age on the saturated lecithin contents of human and rat lung tissues. 7295536 - Comparative lung pathology of rats after exposure to cigarette and cigar smoke. 9950896 - O3-induced inflammation in prepregnant, pregnant, and lactating rats correlates with o3... 14635786 - Thermal hyperalgesia and light touch allodynia after intradermal mycobacterium butyricu... 7279876 - Choline metabolism in placenta: evidence for the biosynthesis of phosphatidylcholine in... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of eukaryotic microbiology Volume: 48 ISSN: 1066-5234 ISO Abbreviation: J. Eukaryot. Microbiol. Publication Date: 2001 Mar-Apr |
Date Detail:
|
Created Date: 2002-07-03 Completed Date: 2002-07-11 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9306405 Medline TA: J Eukaryot Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 182-7 Citation Subset: IM |
Affiliation:
|
Department of Biological Sciences, University of Cincinnati, Ohio 45221, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Coenzymes Female Lovastatin / pharmacology Lung / metabolism, microbiology Mevalonic Acid / metabolism Parabens Phosphoenolpyruvate / metabolism Pneumocystis / isolation & purification, metabolism* Pneumonia, Pneumocystis / microbiology Rats Rats, Inbred Lew Shikimic Acid / metabolism Tyrosine / metabolism Ubiquinone / analogs & derivatives*, biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 AI28757/AI/NIAID NIH HHS; R01 AI29316/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Coenzymes; 0/Parabens; 1339-63-5/Ubiquinone; 138-59-0/Shikimic Acid; 150-97-0/Mevalonic Acid; 2394-68-5/ubiquinone 8; 303-95-7/ubiquinone 7; 303-97-9/ubiquinone 9; 303-98-0/coenzyme Q10; 55520-40-6/Tyrosine; 73-89-2/Phosphoenolpyruvate; 75330-75-5/Lovastatin; 99-96-7/4-hydroxybenzoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Invasion of different cell types by sporozoites of Eimeria species and effects of monoclonal antibod...
Next Document: Redescription of the rare heterotrichid ciliate, Copemetopus subsalsus Villeneuve-Brachon, 1940.