Document Detail


Pneumococcal neuraminidase A: an essential upper airway colonization factor for Streptococcus pneumoniae.
MedLine Citation:
PMID:  22759312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptococcus pneumoniae colonizes the upper respiratory tract from where the organisms may disseminate systemically to cause life threatening infections. The mechanisms by which pneumococci colonize epithelia are not understood, but neuraminidase A (NanA) has a major role in promoting growth and survival in the upper respiratory tract. In this article we show that mutants of S. pneumoniae D39 deficient in NanA or neuraminidase B (NanB) are abrogated in adherence to three epithelial cell lines, and to primary nasopharyngeal cells. Adherence levels were partly restored by nanA complementation in trans. Enzymic activity of NanA was shown to be necessary for pneumococcal adherence to epithelial cells, and adherence of the nanA mutant was restored to wild-type level by pre-incubation of epithelial cells with Lactococcus lactis cells expressing NanA. Pneumococcal nanA or nanB mutants were deficient in biofilm formation, while expression of NanA on L. lactis or Streptococcus gordonii promoted biofilm formation by these heterologous host organisms. The results suggest that NanA is an enzymic factor mediating adherence to epithelial cells by decrypting receptors for adhesion, and functions at least in part as an adhesin in biofilm formation. Neuraminidase A thus appears to play multiple temporal roles in pneumococcal infection, from adherence to host tissues, colonization, and community development, to systemic spread and crossing of the blood-brain barrier.
Authors:
J L Brittan; T J Buckeridge; A Finn; A Kadioglu; H F Jenkinson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular oral microbiology     Volume:  27     ISSN:  2041-1014     ISO Abbreviation:  Mol Oral Microbiol     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-04     Completed Date:  2012-12-03     Revised Date:  2014-02-17    
Medline Journal Info:
Nlm Unique ID:  101524770     Medline TA:  Mol Oral Microbiol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  270-83     Citation Subset:  D    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / physiology
Bacterial Proteins / genetics,  physiology*
Biofilms / growth & development
Cells, Cultured
Epithelial Cells / microbiology
Humans
Mutation
Nasopharynx / cytology
Neuraminidase / genetics,  physiology*
Pneumococcal Infections / enzymology*
Protein Binding
Receptors, Cell Surface / metabolism
Respiratory System / cytology
Streptococcus pneumoniae / enzymology*,  genetics
Virulence Factors*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Receptors, Cell Surface; 0/Virulence Factors; 0/sialic acid receptor; EC 3.2.1.18/Neuraminidase
Comments/Corrections
Erratum In:
Mol Oral Microbiol. 2012 Oct;27(5):480

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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