| Pneumococcal neuraminidase A: an essential upper airway colonization factor for Streptococcus pneumoniae. | |
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MedLine Citation:
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PMID: 22759312 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Streptococcus pneumoniae colonizes the upper respiratory tract from where the organisms may disseminate systemically to cause life threatening infections. The mechanisms by which pneumococci colonize epithelia are not understood, but neuraminidase A (NanA) has a major role in promoting growth and survival in the upper respiratory tract. In this article we show that mutants of S. pneumoniae D39 deficient in NanA or neuraminidase B (NanB) are abrogated in adherence to three epithelial cell lines, and to primary nasopharyngeal cells. Adherence levels were partly restored by nanA complementation in trans. Enzymic activity of NanA was shown to be necessary for pneumococcal adherence to epithelial cells, and adherence of the nanA mutant was restored to wild-type level by pre-incubation of epithelial cells with Lactococcus lactis cells expressing NanA. Pneumococcal nanA or nanB mutants were deficient in biofilm formation, while expression of NanA on L. lactis or Streptococcus gordonii promoted biofilm formation by these heterologous host organisms. The results suggest that NanA is an enzymic factor mediating adherence to epithelial cells by decrypting receptors for adhesion, and functions at least in part as an adhesin in biofilm formation. Neuraminidase A thus appears to play multiple temporal roles in pneumococcal infection, from adherence to host tissues, colonization, and community development, to systemic spread and crossing of the blood-brain barrier. |
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Authors:
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J L Brittan; T J Buckeridge; A Finn; A Kadioglu; H F Jenkinson |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular oral microbiology Volume: 27 ISSN: 2041-1014 ISO Abbreviation: Mol Oral Microbiol Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-07-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101524770 Medline TA: Mol Oral Microbiol Country: Denmark |
Other Details:
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Languages: eng Pagination: 270-83 Citation Subset: D |
Copyright Information:
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© 2012 John Wiley & Sons A/S. |
Affiliation:
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School of Oral and Dental Sciences, University of Bristol, Bristol, UK School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK Department of Clinical Infection Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK. |
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