| Pneumatic Pressure Bioreactor for Cyclic Hydrostatic Stress Application. Mechanobiology Effects on Periodontal Ligament Cells. | |
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MedLine Citation:
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PMID: 21757574 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A bioreactor system was developed to provide high-amplitude cyclic hydrostatic compressive stress (cHSC) using compressed air mixed commercially as needed to create partial pressures of oxygen and carbon dioxide appropriate for the cells under investigation. Operating pressures up to 300 psi are achievable in this system at a minimum cyclic rate of 0.2 Hz. In this study, ligamentous fibroblasts from human periodontal ligaments (n=6) were compressed on 2 consecutive days at 150 psi for 3 hours each day, and the mRNA for families of extracellular matrix protein and protease isomers was evaluated by real-time PCR array. Several integrins were significantly up-regulated, most notably alpha-3 (6.4-fold), as was SPG7 (12.1-fold). Among the collagens, Col8a1 was highly up-regulated at 53.5-fold, with Col6a1, Col6a2, and Col7a1 also significantly up-regulated 4.4 to 8.5 fold. MMP-1 was the most affected at 122.9-fold up-regulation. MMP-14 likewise increased 17.8 fold with slight reductions for the gelatinases, and a significant increase of TIMP-2 at 5.8 fold. The development of this bioreactor system, and its utility in characterizing PDL fibroblast mechanobiology in intermediate-term testing, holds promise for better simulating the conditions of the musculoskeletal system and the large cyclic compressive stresses it may experience in gait, exertion, and mastication. |
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Authors:
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Karl H Wenger; Ahmed Rifaat El-Awady; Regina L W Messer; Mohamed M Sharawy; Greg White; Carol A Lapp |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-14 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Medical College of Georgia. |
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