Document Detail

Pneumatic pressure bioreactor for cyclic hydrostatic stress application: mechanobiology effects on periodontal ligament cells.
MedLine Citation:
PMID:  21757574     Owner:  NLM     Status:  MEDLINE    
A bioreactor system was developed to provide high-amplitude cyclic hydrostatic compressive stress (cHSC) using compressed air mixed commercially as needed to create partial pressures of oxygen and carbon dioxide appropriate for the cells under investigation. Operating pressures as high as 300 psi are achievable in this system at cyclic speeds of up to 0.2 Hz. In this study, ligamentous fibroblasts from human periodontal ligaments (n = 6) were compressed on two consecutive days at 150 psi for 3 h each day, and the mRNA for families of extracellular matrix protein and protease isoforms was evaluated by real-time PCR array. Several integrins were significantly upregulated, most notably alpha-3 (6.4-fold), as was SPG7 (12.1-fold). Among the collagens, Col8a1 was highly upregulated at 53.5-fold, with Col6a1, Col6a2, and Col7a1 also significantly upregulated 4.4- to 8.5-fold. MMP-1 was the most affected at 122.9-fold upregulation. MMP-14 likewise increased 17.8-fold with slight reductions for the gelatinases and a significant increase of TIMP-2 at 5.8-fold. The development of this bioreactor system and its utility in characterizing periodontal ligament fibroblast mechanobiology in intermediate-term testing hold promise for better simulating the conditions of the musculoskeletal system and the large cyclic compressive stresses joints may experience in gait, exertion, and mastication.
Karl H Wenger; Ahmed R El-Awady; Regina L W Messer; Mohamed M Sharawy; Greg White; Carol A Lapp
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Publication Detail:
Type:  Journal Article     Date:  2011-07-14
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  111     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2012-05-08     Completed Date:  2012-12-05     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1072-9     Citation Subset:  IM    
Department of Orthopaedic Surgery, Georgia Health Sciences University, Augusta, GA 30912, USA.
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MeSH Terms
Cells, Cultured
Collagen / genetics,  metabolism
Extracellular Matrix Proteins / genetics,  metabolism
Fibroblasts / cytology*,  metabolism
Gelatinases / genetics,  metabolism
Gene Expression
Hydrostatic Pressure
Matrix Metalloproteinase 1 / genetics,  metabolism
Matrix Metalloproteinase 14 / genetics,  metabolism
Metalloendopeptidases / genetics,  metabolism
Peptide Hydrolases / genetics,  metabolism
Periodontal Ligament / cytology*,  metabolism
RNA, Messenger / genetics
Tissue Inhibitor of Metalloproteinase-2 / genetics,  metabolism
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/RNA, Messenger; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; 9007-34-5/Collagen; EC 3.4.-/Peptide Hydrolases; EC 3.4.24.-/Gelatinases; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.-/SPG7 protein, human; EC Metalloproteinase 1; EC Metalloproteinase 14

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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