Document Detail


Pluripotential reprogramming of the somatic genome in hybrid cells occurs with the first cell cycle.
MedLine Citation:
PMID:  18065396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fusion of pluripotent embryonic cells with somatic cells results in reprogramming of the somatic cell genome. Oct4-green fluorescent protein (GFP) transgenes that do not contain the proximal enhancer (PE) region are widely used to visualize reprogramming of the somatic to the pluripotent cell state. The temporal onset of Oct4-GFP activation has been found to occur 40-48 hours postfusion. We asked whether activation of the transgene actually reflects activation of the endogenous Oct4 gene. In the current study, we show that activation of an Oct4-GFP transgene that contains the PE region occurs within 22 hours of fusion. In addition, demethylation of the Oct4-GFP transgene and that of the endogenous Oct4 and Nanog genes was found to occur within 24 hours of fusion. As this timing corresponds with the timing of cell cycle completion in embryonic stem cells and fusion hybrids (approximately 22 hours), we postulate that pluripotential reprogramming of the somatic cell genome begins during the first cell cycle after the fusion of a somatic cell with a pluripotent cell and has been completed by day 2 postfusion.
Authors:
Dong Wook Han; Jeong Tae Do; Luca Gentile; Martin Stehling; Hoon Taek Lee; Hans R Schöler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-06
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  26     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-04-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  445-54     Citation Subset:  IM    
Affiliation:
Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Cell Fusion
DNA Methylation
Embryonic Stem Cells / cytology*,  metabolism*
Enhancer Elements, Genetic
Female
Gene Expression Regulation
Genome
Green Fluorescent Proteins / genetics
Hybrid Cells / cytology*,  metabolism*
Mice
Mice, Transgenic
Octamer Transcription Factor-3 / genetics
Pluripotent Stem Cells / cytology*,  metabolism*
Promoter Regions, Genetic
Recombinant Fusion Proteins / genetics
Chemical
Reg. No./Substance:
0/Octamer Transcription Factor-3; 0/Pou5f1 protein, mouse; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

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