Document Detail


Pluripotent Hybrid Cells Contribute to Extraembryonic as well as Embryonic Tissues.
MedLine Citation:
PMID:  20946016     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The restricted gene expression of a differentiated cell can be reversed by forming hybrid with embryonic stem cells (ESCs). The resulting hybrid cells showed not only an ESC-specific marker expression but also a differentiation potential similar to the pluripotent fusion partner. Here, we evaluated whether the tetraploid fusion hybrid cells have a unique differentiation potential compared with diploid pluripotent cells. The first Oct4-GFP-positive cells were observed at day 2 following fusion between ESCs and neurosphere cells (OG2(+/-)/ROSA26(+/-)). Reprogramming efficiency was as high as 94.5% at passage 5 and 96.4% at passage 13. We have found that the tetraploid hybrid cells could form chimera with contribution to placenta after blastocyst injection. This result indicates that the tetraploid pluripotent fusion hybrid cells have wide range of differentiation potential. Therefore, we suggest that once the somatic cells are reprogrammed by fusion with ESCs, the tetraploid hybrid cells contributed to the extraembryonic as well as embryonic tissues.
Authors:
Jeong Tae Do; Hyun Woo Choi; Youngsok Choi; Hans R Schöler
Related Documents :
16477406 - Isolation of cytoplasts from satsuma mandarin (citrus unshiu marc.) and production of a...
7163956 - Rapid complementation method for classifying excision repair-defective xeroderma pigmen...
15575716 - Lentiviral vectors pseudotyped with envelope glycoproteins derived from human parainflu...
2946596 - Studies on the effect of glycoprotein processing inhibitors on fusion of l6 myoblast ce...
19607906 - Antiproliferative effects of selenium compounds in colon cancer cells: comparison of di...
14719876 - Microchemical element imaging of yeast and human cells using synchrotron x-ray micropro...
Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Stem cells and development     Volume:  20     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1063-9     Citation Subset:  IM    
Affiliation:
1 Laboratory of Stem Cell and Developmental Biology, CHA Stem Cell Institute, CHA University , Seoul, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evaluation of Moon-tang on allergic reactions.
Next Document:  Exploring new applications for Rhodiola rosea: can we improve the quality of life of patients with s...