Document Detail

Plumage pigmentation and expression of its regulatory genes during quail development--histochemical analysis using Bh (black at hatch) mutants.
MedLine Citation:
PMID:  12351177     Owner:  NLM     Status:  MEDLINE    
The plumage on the dorsal trunk of normal quail embryos exhibits longitudinal black and brown stripes of pigments produced by melanocytes. However, this pigmentation pattern disappeared in Bh (black at hatch) heterozygous and homozygous embryos because of overall black and brown pigmentation of plumages, respectively. To investigate the mechanisms of the pigment pattern formation of plumage and clarify the roles of the Bh locus in the pattern formation, we examined the expression pattern of genes relating to melanocyte development (Mitf, MelEM antigen, Kitl, Kit and EdnrB2) and melanin pigment production (Dct, Tyrp1, Tyr and Mmp115) in Bh mutant and wild-type embryos throughout development. As a result, we found that MelEM antigen was expressed in melanoblasts committed to produce black pigment before apparent melanogenic gene expression, and that Bh heterozygotes and homozygotes showed abnormal expression patterns of the MelEM antigen. These results indicate that MelEM antigen is a good marker for melanoblasts committed to produce black pigment, and suggests that the Bh locus directs melanocytes to produce eumelanin in proper positions.
Tohru Niwa; Makoto Mochii; Akira Nakamura; Nobuyoshi Shiojiri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mechanisms of development     Volume:  118     ISSN:  0925-4773     ISO Abbreviation:  Mech. Dev.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-27     Completed Date:  2003-05-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  139-46     Citation Subset:  IM    
Department of Biology, Faculty of Science, Shizuoka University, 836 Oya, Shizuoka 422-8529, Japan.
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MeSH Terms
Body Patterning
Chick Embryo
Cloning, Molecular
DNA, Complementary / metabolism
Gene Expression Regulation, Developmental*
In Situ Hybridization
Melanocytes / cytology,  metabolism
Oligonucleotides, Antisense / pharmacology
Pigmentation / genetics*
Polymorphism, Restriction Fragment Length
RNA, Messenger / metabolism
Skin / cytology
Time Factors
Tissue Distribution
Reg. No./Substance:
0/Antigens; 0/DNA, Complementary; 0/Oligonucleotides, Antisense; 0/RNA, Messenger

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