| Ploidy and karyotype of hepatocytes isolated from enzyme-altered foci in two different protocols of multistage hepatocarcinogenesis in the rat. | |
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MedLine Citation:
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PMID: 2563236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ploidy and karyotypes of hepatocytes isolated from the livers of rats subjected to the protocols of Peraino et al. and of Solt and Farber were determined by the examination of such cells in primary culture. A study of 100 or more metaphases from each of five rats on each protocol revealed that 75-80% of gamma-glutamyltranspeptidase-positive (GGT+) hepatocytes isolated from livers of rats in either protocol were diploid, whereas only 23-33% of GGT- cells were diploid. Fifty percent or more of the karyotypes of hepatocytes from livers of rats receiving the Solt-Farber protocol exhibited one or more chromosomal breaks, whereas hepatocytes from livers of rats subjected to the Peraino protocol showed no increase in chromosomal breakage over that in normal controls. These studies demonstrate that the majority of GGT+ cells from altered hepatic foci are diploid and that the greater toxicity of the Solt-Farber protocol over that of Peraino is correlated with marked chromosomal breakage of GGT+ hepatocytes. |
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Authors:
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L Sargent; Y H Xu; G L Sattler; L Meisner; H C Pitot |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Carcinogenesis Volume: 10 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1989 Feb |
Date Detail:
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Created Date: 1989-03-07 Completed Date: 1989-03-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 387-91 Citation Subset: IM |
Affiliation:
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McArdle Laboratory for Cancer Research, Department of Oncology, Medical School, University of Wisconsin, Madison 53706. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromosome Aberrations* Karyotyping Liver Neoplasms / enzymology, genetics* Ploidies* Rats gamma-Glutamyltransferase / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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CA-07175/CA/NCI NIH HHS; CA-22484/CA/NCI NIH HHS; ES-07015/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 2.3.2.2/gamma-Glutamyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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