Document Detail


Ploidy determination of Canadida albicans.
MedLine Citation:
PMID:  391796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dimorphic yeast Candida albicans, as a member of the fungi imperfecti, has been assumed to be in the haploid, or imperfect, state. The deoxyribonucleic acid content of this species has been measured by flow microfluorometry, a technique capable of analyzing single cells. These results were compared with flow microfluorometric deoxyribonucleic acid determinations on a series of strains of Saccharomyces cerevisiae of known ploidy (haploid, diploid, triploid, and tetraploid). These ploidy levels were readily distinguished by the flow microfluorometry procedure. By this criterion, C. albicans was found to contain a diploid amount of deoxyribonucleic acid. Ultraviolet radiation survival and chemical mutagenesis experiments support the conclusion that both clinically isolated and laboratory strains of C. albicans are diploid.
Authors:
A F Olaiya; S J Sogin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  140     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-03-27     Completed Date:  1980-03-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1043-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / analysis,  genetics*
DNA, Fungal / analysis*
Diploidy
Fluorometry / methods
Ploidies*
Saccharomyces cerevisiae / analysis,  genetics
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Fungal
Comments/Corrections

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