Document Detail

Ploidy class-dependent metabolic changes in rat hepatocytes after partial hepatectomy.
MedLine Citation:
PMID:  2415377     Owner:  NLM     Status:  MEDLINE    
Glucose-6-phosphate dehydrogenase (G6PDH), succinate dehydrogenase (SDH) activity and the single-stranded RNA (ssRNA) content of isolated hepatocytes of different ploidy classes from adult male rats have been studied after partial hepatectomy using quantitative cytochemical means. The SDH activity and ssRNA content in all classes of hepatocytes are decreased during the first hours after operation followed by an increase above control values. The increase of both SDH activity and ssRNA content is significant only in the mononuclear diploid (MD) cells but not in the hepatocytes of higher ploidy classes and is related with the mitotic wave at 32 h after hepatectomy. After the mitotic wave, the values quickly return to normal levels. The G6PDH activity does not show any significant change in hepatocytes other than MD cells. In MD cells the G6PDH activity is elevated on a highly significant level up to a maximum value of 3.5 times the control value at 48 h after operation. The G6PDH activity in MD cells is returned to normal values within 14 days after operation. It is concluded that: 1. The MD cells show a distinct metabolic behaviour due to their function as stem cells of liver parenchyma and retain at least some of their fetal characteristics. 2. G6PDH activity is not a transformation-linked discriminant for neoplastic metabolism.
C J Van Noorden; I M Vogels; G Fronik; J M Houtkooper; J James
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  161     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-06     Completed Date:  1986-01-06     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  551-7     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation
Glucosephosphate Dehydrogenase / analysis
Liver / cytology,  enzymology,  metabolism*
Liver Regeneration*
RNA / analysis
Rats, Inbred Strains
Stem Cells / metabolism
Succinate Dehydrogenase / analysis
Reg. No./Substance:
63231-63-0/RNA; EC Dehydrogenase; EC Dehydrogenase

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