| Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells. | |
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MedLine Citation:
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PMID: 20378978 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To evaluate the anticarcinogenic activity of methanol extract of Pleurospermum kamtschaticum (PKE), we assessed its apoptosis-inducing capability in HT-29 colon carcinoma cells. PKE treatment for 2 h reduced cell viability in a dose-related manner, and induced apoptotic morphological changes. Flow cytometric analysis indicated that PKE treatment at 0.05 mg/ml induced early apoptosis in 66.2% of HT-29 cells. Additionally, Bcl-2 expression was substantially reduced in PKE-treated HT-29 cells, increasing the Bax/Bcl-2 ratio. The protein levels of procaspase-9 and procaspase-3 were decreased markedly, reflecting caspase-9 and caspase-3 activation, and resulting PARP cleavage was noted in the PKE-treated HT-29 cells. Furthermore, we detected increased NAG-1 expression in the PKE-treated HT-29 cells. In an in vivo study, intraperitoneal PKE administration suppressed the formation of tumor nodules in the lungs of mice. These results indicate that PKE can serve as a beneficial supplement in the treatment and the prevention of colon cancer. |
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Authors:
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Jin-Eun Kim; Won-Yoon Chung; Kyung-Soo Chun; Chang Ki Lee; Hee-Juhn Park; Won-Bae Kim; Kwang-Kyun Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 74 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-05-06 Completed Date: 2010-08-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 788-92 Citation Subset: IM |
Affiliation:
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Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspase 3 / metabolism Caspase 9 / metabolism Cell Survival / drug effects Cells / metabolism, pathology Colonic Neoplasms / metabolism*, pathology Flow Cytometry HT29 Cells Humans Mitochondria / metabolism*, pathology Poly(ADP-ribose) Polymerases / metabolism bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/bcl-2-Associated X Protein; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
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