Document Detail

Pleural mesothelium lubrication after hyaluronidase, neuraminidase or pronase treatment.
MedLine Citation:
PMID:  23669495     Owner:  NLM     Status:  Publisher    
Coefficient of kinetic friction (μ) of pleural mesothelium has been found to increase markedly after mesothelial blotting and rewetting. This increase disappeared after addition of a solution with hyaluronan or sialomucin, though previous morphological studies showed that only sialomucin occurs in mesothelial glycocalyx. In this research we investigated whether μ of rabbit pleural mesothelium increased after hyaluronidase, neuraminidase or pronase treatment. Hyaluronidase and neuraminidase did not increase μ, though neuraminidase cleaved sialic acid from mesothelial glycocalyx of diaphragm specimens, and removed hystochemical stain of sialic acid from glycocalyx. Sialomucin treated with neuraminidase lowered μ of blotted mesothelium, though less than untreated sialomucin; this feature plus lubrication provided by other molecules could explain why μ did not increase after neuraminidase. Short pronase treatment (in order to affect only glycocalyx proteins) increased μ; this increase was removed by hyaluronan or sialomucin. After pronase treatment μ decreased with increase in sliding velocity, indicating a regime of mixed lubrication, as in blotted mesothelium.
Chiara Sironi; Francesca Bodega; Cristina Porta; Emilio Agostoni
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-5-10
Journal Detail:
Title:  Respiratory physiology & neurobiology     Volume:  -     ISSN:  1878-1519     ISO Abbreviation:  Respir Physiol Neurobiol     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-5-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101140022     Medline TA:  Respir Physiol Neurobiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier B.V.
Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy.
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