Document Detail

Pleural mesothelial cells stimulated by asbestos release chemotactic activity for neutrophils in vitro.
MedLine Citation:
PMID:  2643374     Owner:  NLM     Status:  MEDLINE    
The development of the pleural inflammatory response to asbestos remains poorly defined. Importantly, the role of the pleural mesothelial cell in recruitment of neutrophils to the pleural space is not known. We hypothesized that rabbit pleural mesothelial cells stimulated by asbestos fibers release chemotactic factor(s) for neutrophils. Primary cultures of rabbit pleural mesothelial cells were established, and their purity verified by the presence of keratin and hyaluronic acid mucin. Mesothelial cells in serum-free media, in the presence of 30 micrograms/ml of crocidolite asbestos, released chemotaxins for neutrophils. This activity was not dependent on the type of asbestos fiber or fiber length. It was dose-dependent until 30 micrograms/ml of asbestos. The chemotactic fractions had the ability to increase both directed and random migration of neutrophils. The chemotactic activity was not present in sonicated fractions of unstimulated mesothelial cells, nor in supernates of asbestos fibers alone. Characterization of the chemotactic activity showed that it was heat stable (56 degrees C per 30 min) and sensitive to digestion with trypsin and papain. On Sephadex G-50 chromatography, it had a molecular weight between 6,000 and 9,000. Production of the chemotactic activity was inhibitable by cycloheximide. These results demonstrate that pleural mesothelial cells can actively synthesize a protein fraction with chemotactic activity for neutrophils. Production of this mesothelial cell-derived chemotactic activity for neutrophils may play an important role in the initiation of the inflammatory response of the pleura to asbestos.
V B Antony; C L Owen; K J Hadley
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  139     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-02-15     Completed Date:  1989-02-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  199-206     Citation Subset:  AIM; IM    
Department of Medicine, Veterans Administration Medical Center, Indianapolis, IN 46202.
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MeSH Terms
Cells, Cultured
Chemotactic Factors / secretion*
Chemotaxis, Leukocyte
Cycloheximide / pharmacology
Hot Temperature
Neutrophils / physiology
Pleura / cytology,  drug effects,  metabolism*
Trypsin / pharmacology
Reg. No./Substance:
0/Chemotactic Factors; 0/Interleukin-8; 1332-21-4/Asbestos; 66-81-9/Cycloheximide; EC

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