Document Detail


Pleiotropic roles of polyglycerolphosphate synthase of lipoteichoic acid in growth of Staphylococcus aureus cells.
MedLine Citation:
PMID:  18952789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipoteichoic acid (LTA) is one of two anionic polymers on the surface of the gram-positive bacterium Staphylococcus aureus. LTA is critical for the bacterium-host cell interaction and has recently been shown to be required for cell growth and division. To determine additional biological roles of LTA, we found it necessary to identify permissive conditions for the growth of an LTA-deficient mutant. We found that an LTA-deficient S. aureus Delta ltaS mutant could grow at 30 degrees C but not at 37 degrees C. Even at the permissive temperature, Delta ltaS mutant cells had aberrant cell division and separation, decreased autolysis, and reduced levels of peptidoglycan hydrolases. Upshift of Delta ltaS mutant cells to a nonpermissive temperature caused an inability to exclude Sytox green dye. A high-osmolarity growth medium remarkably rescued the colony-forming ability of the Delta ltaS mutant at 37 degrees C, indicating that LTA synthesis is required for growth under low-osmolarity conditions. In addition, the Delta ltaS mutation was found to be synthetically lethal with the Delta tagO mutation, which disrupts the synthesis of the other anionic polymer, wall teichoic acid (WTA), at 30 degrees C, suggesting that LTA and WTA compensate for one another in an essential function.
Authors:
Yusuke Oku; Kenji Kurokawa; Miki Matsuo; Sakuo Yamada; Bok-Luel Lee; Kazuhisa Sekimizu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-24
Journal Detail:
Title:  Journal of bacteriology     Volume:  191     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-02-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  141-51     Citation Subset:  IM    
Affiliation:
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
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MeSH Terms
Descriptor/Qualifier:
DNA Primers
Gene Amplification
Genetic Vectors
Glycerophosphates / biosynthesis
Ligases / metabolism*
Lipopolysaccharides / biosynthesis*,  deficiency
Mutation
Organic Chemicals / metabolism
Polymerase Chain Reaction
Staphylococcus aureus / enzymology,  genetics*,  growth & development
Teichoic Acids / biosynthesis*
Temperature
Thermodynamics
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Glycerophosphates; 0/Lipopolysaccharides; 0/Organic Chemicals; 0/SYTOX Green; 0/Teichoic Acids; 52007-79-1/polyglycerolphosphate; 56411-57-5/lipoteichoic acid; EC 6.-/Ligases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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