| Pleiotropic effects of the goat prolactin receptor genotype on milk fatty acid composition. | |
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MedLine Citation:
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PMID: 20399061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the lactating mammary gland, prolactin (PRL) stimulates the synthesis of lactose as well as fatty acid uptake, lipogenesis, and triacylglycerol synthesis. Associations between bovine PRL receptor (PRLR) genotype and fat yield have been reported, which illustrates the role of PRL in conveying lipids toward the udder as well as in stimulating their local synthesis during lactation. Conversely, and to the best of our knowledge, the effects of PRLR genotype on milk fatty acid content have not been studied so far in any mammalian species. In this study, we sequenced most of the coding region of the caprine PRLR gene in several individuals from the Malagueña and Murciano-Granadina breeds. This approach allowed us to identify 2 long and short mRNA isoforms, produced by alternative splicing, and 4 single-nucleotide polymorphisms (SNPs), namely, c.177T>C, c.1131G>A, c.1201G>A and c.1355C>T. Two of these SNPs are nonsynonymous and involve G401R (c.1201G>A) and T452I (c.1355C>T) amino acid substitutions in the cytoplasmic domain of PRLR, which plays a fundamental role in signal transduction. Performance of an association analysis with milk composition traits in a Murciano-Granadina goat population revealed highly suggestive effects on palmitoleic acid content, whereas suggestive effects were detected for other fatty acids, such as palmitic and linoleic. These results are consistent with the pleiotropic effects of PRL on mammary gland lipid metabolism and milk composition. |
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Authors:
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A Zidi; J M Serradilla; J Jordana; J Carrizosa; B Urrutia; O Polvillo; P González-Redondo; D Gallardo; Marcel Amills; Víctor Manuel Fernández-Cabanás |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-26 |
Journal Detail:
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Title: Domestic animal endocrinology Volume: 39 ISSN: 1879-0054 ISO Abbreviation: Domest. Anim. Endocrinol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-06 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8505191 Medline TA: Domest Anim Endocrinol Country: United States |
Other Details:
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Languages: eng Pagination: 85-89.e2 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Bellaterra, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing Animals Fatty Acids / analysis, metabolism* Fatty Acids, Monounsaturated / analysis, metabolism Female Genetic Association Studies Genetic Variation Goats Lactation / genetics*, metabolism Linoleic Acid / analysis, metabolism Mammary Glands, Animal / metabolism* Milk / chemistry*, metabolism Palmitic Acid / analysis, metabolism Polymorphism, Single Nucleotide Protein Isoforms RNA, Messenger / analysis* Receptors, Prolactin / classification, genetics*, metabolism Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Prolactin; 2091-29-4/palmitoleic acid; 2197-37-7/Linoleic Acid; 57-10-3/Palmitic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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