Document Detail

Pleiotropic effects of the goat prolactin receptor genotype on milk fatty acid composition.
MedLine Citation:
PMID:  20399061     Owner:  NLM     Status:  MEDLINE    
In the lactating mammary gland, prolactin (PRL) stimulates the synthesis of lactose as well as fatty acid uptake, lipogenesis, and triacylglycerol synthesis. Associations between bovine PRL receptor (PRLR) genotype and fat yield have been reported, which illustrates the role of PRL in conveying lipids toward the udder as well as in stimulating their local synthesis during lactation. Conversely, and to the best of our knowledge, the effects of PRLR genotype on milk fatty acid content have not been studied so far in any mammalian species. In this study, we sequenced most of the coding region of the caprine PRLR gene in several individuals from the Malagueña and Murciano-Granadina breeds. This approach allowed us to identify 2 long and short mRNA isoforms, produced by alternative splicing, and 4 single-nucleotide polymorphisms (SNPs), namely, c.177T>C, c.1131G>A, c.1201G>A and c.1355C>T. Two of these SNPs are nonsynonymous and involve G401R (c.1201G>A) and T452I (c.1355C>T) amino acid substitutions in the cytoplasmic domain of PRLR, which plays a fundamental role in signal transduction. Performance of an association analysis with milk composition traits in a Murciano-Granadina goat population revealed highly suggestive effects on palmitoleic acid content, whereas suggestive effects were detected for other fatty acids, such as palmitic and linoleic. These results are consistent with the pleiotropic effects of PRL on mammary gland lipid metabolism and milk composition.
A Zidi; J M Serradilla; J Jordana; J Carrizosa; B Urrutia; O Polvillo; P González-Redondo; D Gallardo; Marcel Amills; Víctor Manuel Fernández-Cabanás
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-26
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  39     ISSN:  1879-0054     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-06     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  85-89.e2     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Bellaterra, Spain.
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MeSH Terms
Alternative Splicing
Fatty Acids / analysis,  metabolism*
Fatty Acids, Monounsaturated / analysis,  metabolism
Genetic Association Studies
Genetic Variation
Lactation / genetics*,  metabolism
Linoleic Acid / analysis,  metabolism
Mammary Glands, Animal / metabolism*
Milk / chemistry*,  metabolism
Palmitic Acid / analysis,  metabolism
Polymorphism, Single Nucleotide
Protein Isoforms
RNA, Messenger / analysis*
Receptors, Prolactin / classification,  genetics*,  metabolism
Species Specificity
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Prolactin; 2091-29-4/palmitoleic acid; 2197-37-7/Linoleic Acid; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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