Document Detail

Platelets stimulate expression of endothelin mRNA and endothelin biosynthesis in cultured endothelial cells.
MedLine Citation:
PMID:  1873876     Owner:  NLM     Status:  MEDLINE    
Modulation of the biosynthesis of the vasoconstrictor peptide endothelin was studied in cultured endothelial cells. Immunoreactive endothelin (irET) levels were significantly elevated in conditioned medium from bovine pulmonary artery endothelial (BPAE) or human umbilical vein endothelial cells when coincubated with washed human platelets. Platelets (approximately 200,000 cells/microliters) enhanced irET levels approximately 250% over basal levels. Stimulation of irET levels in BPAE cell-conditioned medium by platelets was time and platelet number dependent. Platelets, as well as thrombin and transforming growth factor-beta 1, stimulated the expression of preproendothelin-1 mRNA in a time-dependent manner. Coincubation of low doses of thrombin (0.1 unit/ml) and subthreshold concentrations of platelets with BPAE cells resulted in a further enhancement of irET levels in conditioned medium. Platelet-mediated stimulation of irET production was not significantly affected by indomethacin (1 microM) or the platelet-activating factor receptor antagonist WEB 2086 (1 microM); however, coincubation of endotoxin (100 ng/ml) with platelets and BPAE cells resulted in significantly higher levels of irET. Whether direct contact or adhesion between platelets and endothelial cells is necessary for stimulating irET release was studied by separating platelets from BPAE cells with a 0.4 microns permeable membrane. Under these conditions, platelets still produced significant elevations (approximately 190% over basal levels) in irET levels in BPAE cell-conditioned medium. In addition, platelet-free buffer from agonist-induced platelet aggregation also significantly enhanced irET production (200% over basal values). These data indicate that a platelet-derived regulatory factor can induce the biosynthesis of endothelin from cultured endothelial cells and also suggest that platelets might play a role in vasomotor regulation via a novel intercellular interaction with the endothelium.
E H Ohlstein; B L Storer; J A Butcher; C Debouck; G Feuerstein
Related Documents :
16009346 - Downregulation of the angiogenesis inhibitor thrombospondin 1 in fibroblasts exposed to...
1520706 - Gangliosides inhibit platelet-derived growth-factor-stimulated increases in intracellul...
17177826 - Differential expression of platelet-derived growth factor receptors in porcine fibrobla...
1725286 - Lyophilized type-i collagen and chronic leg ulcers.
24188486 - Inflammatory biomarkers and atrial fibrillation: potential role of inflammatory pathway...
20979176 - Development of antibodies to human thrombin and factor v in a pediatric patient exposed...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Circulation research     Volume:  69     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-09-26     Completed Date:  1991-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  832-41     Citation Subset:  IM    
Department of Pharmacology, SmithKline Beecham Research Laboratories, King of Prussia, Pa. 19406-0939.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Platelets / drug effects,  physiology*
Blotting, Northern
Cells, Cultured
Culture Media
Endothelins / analysis,  biosynthesis*,  genetics*
Endothelium, Vascular / cytology,  metabolism*
Endotoxins / pharmacology
Indomethacin / pharmacology
Pulmonary Artery
RNA, Messenger / analysis*
Thrombin / pharmacology
Umbilical Veins
Reg. No./Substance:
0/Culture Media; 0/Endothelins; 0/Endotoxins; 0/RNA, Messenger; 53-86-1/Indomethacin; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Dose-dependent inhibition of stretch-induced arrhythmias by gadolinium in isolated canine ventricles...
Next Document:  Optical recordings in the rabbit heart show that defibrillation strength shocks prolong the duration...