Document Detail

Platelet thrombus formation and hemostasis are delayed in the microcirculation of copper-deficient rats.
MedLine Citation:
PMID:  8064374     Owner:  NLM     Status:  MEDLINE    
The role of dietary copper in platelet thrombus formation and hemostasis was studied in the cremaster muscle microcirculation. Male weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (94 mumol Cu/kg diet) or copper-deficient (0 mumol Cu/kg diet) for 1, 3 or 5 wk. The rats were anesthetized with pentobarbital, and the cremaster was spread in a Krebs-filled tissue bath. Fluorescein isothiocyanate tagged to bovine serum albumin (FITC-BSA) was injected intra-arterially. After a 20-min equilibration, blue light (1.8 W/cm2, 450-490 nm) was used to activate the FITC-BSA and induced platelet thrombus formation within the vasculature. In vivo television microscopy was used to quantify the thrombus formation. In rats fed the copper-deficient diet for 3 or 5 wk, platelet thrombus formation induced by photoactivation was significantly (P < 0.05) delayed and prothrombin time was significantly longer but the number of circulating platelets was significantly greater than in age-matched rats fed the copper-adequate diet. Bleeding time, measured after micropuncture of a second-order venule, was significantly longer but hematocrit was significantly lower in rats fed the copper-deficient diet than in those fed the copper-adequate diet. The results demonstrate that platelet-mediated hemostasis is depressed in dietary copper deficiency and that this may be due to a decrease in hematocrit, a decrease in the activity of a coagulation factor and/or an alteration of platelet function.
D A Schuschke; J T Saari; J W Nuss; F N Miller
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of nutrition     Volume:  124     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-09-19     Completed Date:  1994-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1258-64     Citation Subset:  IM    
Center for Applied Microcirculatory Research, University of Louisville, KY 40292.
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MeSH Terms
Bleeding Time
Blood Platelets / physiology*
Copper / administration & dosage,  deficiency*,  physiology
Fluorescein-5-isothiocyanate / analogs & derivatives,  pharmacology
Microcirculation / physiopathology*
Platelet Count
Prothrombin Time
Rats, Sprague-Dawley
Serum Albumin, Bovine / pharmacology
Reg. No./Substance:
0/Serum Albumin, Bovine; 0/fluorescein isothiocyanate bovine serum albumin; 3326-32-7/Fluorescein-5-isothiocyanate; 7440-50-8/Copper

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