| Platelet-rich plasma protects rotator cuff-derived cells from the deleterious effects of triamcinolone acetonide. | |
| | |
MedLine Citation:
|
PMID: 23280560 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Triamcinolone acetonide (TA) injections are widely used to treat enthesopathy, but they may induce adverse effects such as tendon impairment and rupture. Platelet-rich plasma (PRP) is a blood fraction containing high platelet concentrations and various growth factors that play a role in tissue repair processes. The purpose of this study is to investigate whether TA has deleterious effects on human rotator cuff-derived cells, and if PRP can protect these cells from the effects of TA. Human rotator cuff-derived cells were cultured with and without TA and PRP, and the culture without any additive served as the control. Cell morphology was assessed at days 7 and 21. Cell viability was evaluated at days 1, 7, 14, and 21 by a water-soluble tetrazolium salt assay. Induction of apoptosis was measured by immunofluorescence staining and flow cytometry at day 7. Induction of cleaved caspase-3 was measured by immunofluorescence staining at day 7. The cells cultured with TA had a flattened and polygonal shape at day 7. The cells cultured with both TA and PRP were similar in appearance to control cells. Exposure to TA also significantly decreased cell viability, but cell viability did not decrease when PRP was added along with TA. The number of apoptotic cells increased with TA exposure, while addition of PRP prevented cell apoptosis. In conclusion, the deleterious effect of TA was prevented by PRP, which can be used as a protective agent for patients receiving local TA injections. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. |
| | |
Authors:
|
Tomoyuki Muto; Takeshi Kokubu; Yutaka Mifune; Ryosuke Sakata; Issei Nagura; Hanako Nishimoto; Yoshifumi Harada; Kotaro Nishida; Ryosuke Kuroda; Masahiro Kurosaka |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-31 |
Journal Detail:
|
Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: - ISSN: 1554-527X ISO Abbreviation: J. Orthop. Res. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2013-1-2 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Orthopaedic Research Society. |
Affiliation:
|
Department of Orthopaedic Surgery, Kobe University, Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Partial volume correction in arterial spin labeling using a Look-Locker sequence.
Next Document: Phaco-efficiency test and re-aspiration analysis of repulsed particle in phacoemulsification.