Document Detail

Platelet-rich plasma has no effect on increasing free fat graft survival in the nude mouse.
MedLine Citation:
PMID:  18550460     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Free fat grafts have an unpredictable survival rate, which may be dependent on host bed vascularity. Therefore, the authors hypothesized that the presence of growth factors in platelet-rich plasma (PRP), may enhance free fat graft survival. METHODS: Free fat grafts and autologous PRP were harvested from a healthy female and processed using the Coleman technique and the Medtronic Magellan system respectively. The experiment comprised two groups of 12 nude mice each with injection of free fat grafts into the scalp. The experimental group comprised the combination of 0.8 ml of free fat graft and 0.2 ml of PRP. The control group comprised the combination of 0.8 ml of free fat graft and 0.2 ml of normal saline. The mice were euthanized after 16 weeks and the fat grafts explanted and measured for weight and volume. Histology was performed with Oil Red O stain. Statistical analysis of the weight and volume in between groups was performed using the independent samples T-test (SPSS v11). The Mann-Whitney test was used to compare the ranking of six histological parameters between the two groups. RESULTS: The mean weight and volume for the experimental arm were 0.503 g and 0.545 ml respectively. The mean weight and volume for the control arm were 0.500 g and 0.541 ml respectively. The weight, volume and histological parameters between the two groups were not statistically significant. A mouse from each group died of unknown causes. CONCLUSION: PRP did not enhance free fat graft survival in the nude mouse.
Yong-Chen Por; Vincent Kok-Leng Yeow; Nayef Louri; Tony Kiat-Hon Lim; Irene Kee; In-Chin Song
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-11
Journal Detail:
Title:  Journal of plastic, reconstructive & aesthetic surgery : JPRAS     Volume:  62     ISSN:  1878-0539     ISO Abbreviation:  -     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-17     Completed Date:  2009-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264239     Medline TA:  J Plast Reconstr Aesthet Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1030-4     Citation Subset:  IM    
Department of Plastic Surgery, Kandang Kerbau Women's and Children's Hospital, Singapore.
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MeSH Terms
Adipocytes / transplantation*
Graft Survival / physiology*
Mice, Nude
Platelet-Rich Plasma / physiology*

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