| Platelet sarco/endoplasmic reticulum Ca2+ATPase isoform 3b and Rap 1b: interrelation and regulation in physiopathology. | |
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MedLine Citation:
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PMID: 9576865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Platelet Ca2+ signalling involves intracellular Ca2+ pools, whose content is controlled by sarco/endoplasmic reticulum Ca2+ATPases (SERCAs). Among these, a key role is played by the inositol trisphosphate-sensitive Ca2+ pool, associated with the SERCA 3b isoform. We have investigated the control of this Ca2+ pool through the cAMP-dependent phosphorylation of the GTP-binding protein, Rap (Ras-proximate) 1b. We first looked for this Ca2+ pool target of regulation by studying the expression of the different SERCA and Rap 1 proteins in human platelets and various cell lines, by Western blotting and reverse transcription-PCR. Since co-expression of Rap 1b and SERCA 3b was obtained, we looked for their protein-protein interaction as a function of the cAMP-dependent phosphorylation of Rap 1b. Co-immunoprecipitations of SERCA 3b and Rap 1b proteins were found in the absence of phosphorylation, induced by the catalytic subunit of the cAMP-dependent protein kinase (csPKA). In contrast, upon pre-treatment of platelet membranes with csPKA, the SERCA 3b dissociated from the Rap 1b protein, in agreement with a role of its phosphorylated state in their interaction. Finally, we looked for adaptation of this complex in a platelet pathological model of hypertension. We investigated the expression of both proteins, as well as the cAMP-dependent phosphorylation of Rap 1b and SERCA 3b activity in platelets from control normotensive Wistar-Kyoto rats and from spontaneously hypertensive rats (SHRs). A decrease in SERCA 3b activity was associated with a decrease in Rap 1b endogenous phosphorylation in SHR platelets, consistent with a functional role in the regulation of the SERCA 3b-associated Ca2+ pool. |
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Authors:
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C Lacabaratz-Porret; E Corvazier; T Kovàcs; R Bobe; R Bredoux; S Launay; B Papp; J Enouf |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 332 ( Pt 1) ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1998 May |
Date Detail:
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Created Date: 1998-08-17 Completed Date: 1998-08-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 173-81 Citation Subset: IM |
Affiliation:
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Institut National de la Santé et de la Recherche Médicale U 348, IFR Circulation Lariboisière, Hôpital Lariboisière, 8 rue Guy Patin, 75475 Paris Cedex 10, France. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Platelets / enzymology* Calcium-Transporting ATPases / metabolism* Cell Line Cyclic AMP / pharmacology Endoplasmic Reticulum / enzymology* GTP-Binding Proteins / physiology* Gene Expression Regulation, Enzymologic / physiology Humans Hypertension / physiopathology Inositol Phosphates / physiology Isoenzymes / metabolism Phosphorylation RNA, Messenger / metabolism Rats Rats, Inbred SHR Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 0/Isoenzymes; 0/RNA, Messenger; 60-92-4/Cyclic AMP; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.1.8/Calcium-Transporting ATPases |
| Comments/Corrections | |
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