Document Detail


Platelet interaction with pyrolytic carbon heart-valve leaflets.
MedLine Citation:
PMID:  8884503     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the newest generation of mechanical heart-valve prosthetics constructed either partially or wholly of lowtemperature isotropic pyrolytic carbon (LTIC) have significantly reduced thromboembolic complications compared with early-generation mechanical valves (e.g., Starr-Edwards), thromboembolism remains an important clinical complication. In the present study, high-resolution, lowvoltage scanning electron microscopy (HR-LV-SEM) was used to examine the structure and platelet interaction properties of LTIC valve leaflets manufactured by both Carbo Medics, Inc. and by St. Jude Medical, Inc. Valve leaflets from both manufacturers, prepared and polished exactly as used in clinical heart valves, had similar surface energetics and elemental composition. Examination with LV-SEM revealed a rough and complex three-dimensional surface structure with nanometer- to micron-size features. In vitro adhesion of human platelets on the LTIC materials and Formvar were evaluated in the presence of 1 mg/mL albumin. Platelet-surface activation, as evaluated by shape change, spread area, and deposition, was extremely extensive on the LTIC materials compared with the Formvar positive control material. LTIC-adherent platelets were extremely thin, and closely followed the rough LTIC contours, greatly limiting their visibility with conventional SEM. These observations demonstrate that LTIC surfaces can extensively activate platelets even in the presence of albumin, thereby suggesting that platelet interactions with pyrolytic carbon may have a significant role in mechanical-valve thromboembolism.
Authors:
S L Goodman; K S Tweden; R M Albrecht
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  32     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-18     Completed Date:  1997-02-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  249-58     Citation Subset:  IM    
Affiliation:
Dept. Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Biocompatible Materials*
Blood Platelets / physiology*,  ultrastructure
Carbon*
Cell Membrane / physiology,  ultrastructure
Heart Valve Prosthesis*
Hot Temperature
Humans
Microscopy, Electron, Scanning
Platelet Adhesiveness*
Grant Support
ID/Acronym/Agency:
HL-37351/HL/NHLBI NIH HHS; RR-570/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/pyrolytic carbon; 7440-44-0/Carbon

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