| Platelet function is inhibited by nitric oxide liberation during nitroglycerin-induced hypotension anaesthesia. | |
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MedLine Citation:
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PMID: 9389267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The inhibitory effects of nitroglycerin (NTG) on platelet function and the mechanisms of inhibition have been studied in vitro, but not in vivo. Therefore, we have investigated the effects of NTG on platelet function in eight patients undergoing orthopaedic surgery. Simultaneous measurements of platelet aggregation and change in intracellular calcium concentrations were performed in Fura-2 loaded platelets using thrombin as a stimulator. Intraplatelet concentrations of cyclic 3',5'-guanine monophosphate (cGMP) were measured by radioimmunoassay, and the concentration of nitrite ion was also measured. Continuous i.v. infusion of NTG 4-8 micrograms kg-1 min-1 significantly inhibited platelet aggregation and the increase in intracellular Ca2+ concentration (first phase, mean 439.9 (SEM 68.7) vs 210.6 (38.7) nmol litre-1; second phase, 154.4 (19.8) vs 106.7 (18.0) nmol litre-1). The concentration of cGMP (from 0.633 (0.098) to 1.764 (0.578) pmol/10(9) platelets) and the concentration of nitrite ion (from 532.6 (17.6) to 724.4 (34.8) nmol litre-1) also increased significantly after infusion of NTG. The NTG concentration in plasma was of the order of 10(-8) mol litre-1. We have demonstrated that in vivo, NTG increased intraplatelet cGMP concentrations and inhibited platelet function; one mechanisms of this effect is likely to be related to nitric oxide liberation from NTG bioconversion. |
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Authors:
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H Aoki; M Inoue; T Mizobe; M Harada; H Imai; A Kobayashi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: British journal of anaesthesia Volume: 79 ISSN: 0007-0912 ISO Abbreviation: Br J Anaesth Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1997-12-17 Completed Date: 1997-12-17 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372541 Medline TA: Br J Anaesth Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 476-81 Citation Subset: IM |
Affiliation:
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Department of Anaesthesiology, Kyoto Prefectural University of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Anesthesia, General* Bleeding Time Blood Platelets / drug effects*, physiology Calcium / blood Cyclic GMP / blood Female Humans Hypotension, Controlled* Male Middle Aged Nitric Oxide / physiology Nitroglycerin / blood, pharmacology* Platelet Aggregation / drug effects Platelet Aggregation Inhibitors / pharmacology* Vasodilator Agents / blood, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Platelet Aggregation Inhibitors; 0/Vasodilator Agents; 10102-43-9/Nitric Oxide; 55-63-0/Nitroglycerin; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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