Document Detail

Platelet-derived growth factor signals play critical roles in differentiation of longitudinal smooth muscle cells in mouse embryonic gut.
MedLine Citation:
PMID:  18194151     Owner:  NLM     Status:  MEDLINE    
In the development of mouse gut, longitudinal smooth muscle cells (LMC) and interstitial cells of Cajal (ICC) originate from common precursor cells expressing c-Kit. Recently, some gastrointestinal stromal tumours, which develop from smooth muscle layers of the gut and have gain-of-function mutations of c-kit, have been reported to have gain-of-function mutations of platelet-derived growth factor (PDGF) receptor alpha gene. These data raise the possibility that PDGF signalling might be involved in the development of LMC. Therefore, we examined the expression pattern of the PDGF signal family of embryonic gut by immunohistochemistry and in situ hybridization, and investigated the role of PDGF signals in the development of smooth muscle layers in mouse gut using a new organ culture system. During embryonic development, the circular muscle layer expressed PDGF-A, enteric neurons expressed PDGF-B and common precursor cells of LMC and ICC expressed both PDGF receptor alpha and beta. The selective PDGF receptor inhibitor AG1295 suppressed the differentiation of LMC in gut explants. We conclude that PDGF signals play critical roles in the differentiation of LMC in mouse embryonic gut.
M Kurahashi; Y Niwa; J Cheng; Y Ohsaki; A Fujita; H Goto; T Fujimoto; S Torihashi
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-10
Journal Detail:
Title:  Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society     Volume:  20     ISSN:  1365-2982     ISO Abbreviation:  Neurogastroenterol. Motil.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-17     Completed Date:  2008-08-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9432572     Medline TA:  Neurogastroenterol Motil     Country:  England    
Other Details:
Languages:  eng     Pagination:  521-31     Citation Subset:  IM    
Department of Anatomy & Molecular Cell Biology, Nagoya University Graduate School of Medicine, Tsurumai, Showa-ku, Nagoya, Japan.
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MeSH Terms
Cell Differentiation* / physiology
Gastrointestinal Tract / cytology*,  embryology*,  physiology
Mice, Inbred BALB C
Myocytes, Smooth Muscle / cytology*,  physiology
Organ Culture Techniques
Platelet-Derived Growth Factor / physiology*
Receptors, Platelet-Derived Growth Factor / physiology
Signal Transduction* / physiology
Reg. No./Substance:
0/Platelet-Derived Growth Factor; EC, Platelet-Derived Growth Factor

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