| Platelet-derived growth factor promotes the proliferation of human umbilical cord-derived mesenchymal stem cells. | |
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MedLine Citation:
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PMID: 22961649 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study was designed to investigate the effect of platelet-derived growth factor (PDGF) on the proliferation of human umbilical cord mesenchymal stem cells (UC-MSCs) and further explore the mechanism of PDGF in promoting the proliferation of UC-MSCs. The human UC-MSCs were treated with different concentrations of PDGF, and the effects were evaluated by counting the cell number, the cell viability, the expression of PDGF receptors analyzed by RT-PCR, and the detection of the gene expression of cell proliferation, cell cycle and pluripotency, and Brdu assay by immunofluorescent staining and Quantitative real-time (QRT-PCR). The results showed that PDGF could promote the proliferation of UC-MSCs in vitro in a dose-dependent way, and 10 to 50 ng/ml PDGF had a significant proliferation effect on UC-MSCs; the most obvious concentration was 50 ng/ml. Significant inhibition on the proliferation of UC-MSCs was observed when the concentration of PDGF was higher than 100 ng/ml, and all cells died when the concentration reached 200 ng/ml PDGF. The PDGF-treated cells had stronger proliferation and antiapoptotic capacity than the control group by Brdu staining. The expression of the proliferation-related genes C-MYC, PCNA and TERT and cell cycle-related genes cyclin A, cyclin 1 and CDK2 were up-regulated in PDGF medium compared with control. However, pluripotent gene OCT4 was not significantly different between cells cultured in PDGF and cells analyzed by immunofluorescence and QRT-PCR. The PDGF could promote the proliferation of human UC-MSCs in vitro. Copyright © 2012 John Wiley & Sons, Ltd. |
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Authors:
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Pubin Qiu; Wencong Song; Zhiwei Niu; Yaofu Bai; Wei Li; Shaohui Pan; Sha Peng; Jinlian Hua |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-7 |
Journal Detail:
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Title: Cell biochemistry and function Volume: - ISSN: 1099-0844 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 John Wiley & Sons, Ltd. |
Affiliation:
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College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Key Laboratory of Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling 712100, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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