| Platelet-derived growth factor-D expression in developing and mature human kidneys. | |
| | |
MedLine Citation:
|
PMID: 12427128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules composed of sulfide-bonded dimeric structures. Two well-studied PDGF peptides (PDGF-A and PDGF-B) have been shown to mediate a wide range of biological effects. PDGF-D is a newly recognized member of the PDGF family. Initial studies of the PDGF-D gene found its expression in cells of the vascular wall, suggesting that it could participate in vascular development and pathology. However, its localization in human kidney tissues has never been studied. METHODS: PDGF-D expression in fetal (N = 30) and adult (N = 25) human kidney tissues was examined by immunohistochemistry using an affinity-purified antibody raised to human PDGF-D. Antibody absorption with the immunizing peptide was employed to confirm the specificity of this antibody. PDGF-D protein and gene expression in human kidneys also were demonstrated by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: In the developing kidney, PDGF-D was first expressed by epithelial cells of comma- and S-shaped structures of the developing nephron, and most consistently in the visceral epithelial cells in the later stages of glomerular differentiation. In addition, PDGF-D could be found in mesenchymal, presumptively fibroblast cells in the interstitium of developing renal pelvis and in fetal smooth muscle cells in arterial vessels. In the adult normal kidney, PDGF-D was expressed by the visceral epithelial cells. There was persistent expression in arterial smooth muscle cells as well as in some neointimal smooth muscle cells of arteriosclerotic vessels, and expression in smooth muscle cells of vasa rectae in the medulla. PDGF-D could be identified at the basolateral membrane of some injured tubules in areas of chronic tubulointerstitial injury routinely encountered in aging kidneys. Western blotting of homogenates of adult kidneys demonstrated monospecific bands at 50 kD corresponding to previously established size parameter for this protein. RT-PCR of human kidney RNA resulted in a 918 basepair band, the sequence of which corresponded to human PDGF-D (Genbank number AF336376). CONCLUSIONS: To our knowledge, these are the first studies to localize PDGF-D in human kidneys and suggest that PDGF-D may have a role in kidney development. PDGF-D was shown to bind to PDGF beta receptor, which localizes to mesangial cells, parietal epithelial cells, and interstitial fibroblasts, suggesting potential paracrine interactions between those cells and the visceral epithelium. |
| | |
Authors:
|
Siribha Changsirikulchai; Kelly L Hudkins; Tracy A Goodpaster; John Volpone; Stavros Topouzis; Debra G Gilbertson; Charles E Alpers |
Related Documents
:
|
6601138 - A thymocyte-activating factor derived from glomerular mesangial cells. 15614148 - The effect of alpha-melanocyte-stimulating hormone on renal tubular cell apoptosis and ... 20065948 - Inhibiting nonmuscle myosin ii impedes inflammatory infiltration and ameliorates progre... 15012698 - A mechanism for the development of subepithelial deposits in a patient with type iii me... 2412978 - Importance of coordinated immune stimulation in experimental antitumor treatment. 12719628 - Il-10 and glucocorticoids inhibit abeta(1-42)- and lipopolysaccharide-induced pro-infla... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Kidney international Volume: 62 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 2002 Dec |
Date Detail:
|
Created Date: 2002-11-12 Completed Date: 2003-05-23 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: United States |
Other Details:
|
Languages: eng Pagination: 2043-54 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Srinakharinwirot University, Bangkok, Thailand. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/AF336376 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Antibody Specificity Blotting, Western Fetus / chemistry Gene Expression Regulation, Developmental Humans Immunohistochemistry Kidney / chemistry*, embryology*, physiology Lymphokines* Molecular Sequence Data Platelet-Derived Growth Factor / analysis*, genetics, immunology RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
|
DK47959/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Lymphokines; 0/PDGFD protein, human; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dual effects of Escherichia coli alpha-hemolysin on rat renal proximal tubule cells.
Next Document: Cyclosporine A-induced hypercalciuria in calbindin-D28k knockout and wild-type mice.