Document Detail

Platelet-derived growth factor-BB supports the survival of cultured rat Schwann cell precursors in synergy with neurotrophin-3.
MedLine Citation:
PMID:  10756078     Owner:  NLM     Status:  MEDLINE    
To understand the intimate axon-Schwann cell relationship required for the accurate development and regeneration of the peripheral nervous system (PNS), it is important to elucidate the repertoire of growth factors involved in this tightly regulated bi-directional dialogue. We focused on the identification and functional characterization of receptor tyrosine kinases (RTKs) in Schwann cells to gain insights into the corresponding growth factor ligands, which may be regulating the highly controlled differentiation of the Schwann cell lineage. Using an RT-PCR based differential display approach, we have identified 17 tyrosine kinases in embryonic rat sciatic nerves during the crucial transition from Schwann cell precursors to early Schwann cells. In this study, we have examined the expression and function of TrkC and the platelet-derived growth factor (PDGF) receptors alpha and beta on Schwann cell precursor cells. These receptors are expressed on freshly isolated Schwann cell precursors, and we show that PDGF-BB is able to rescue a subpopulation of these cells from apoptotic cell death in vitro. Furthermore, the TrkC-ligand neurotrophin-3 (NT-3) can act synergistically to potentiate this effect. However, PDGF-BB and NT-3 do not induce Schwann cell precursor proliferation or differentiation. Our data are consistent with a model suggesting that a combination of growth factors that include PDGF-BB and NT-3 are acting in concert and in synergy to regulate early Schwann cell development.
C S Lobsiger; B Schweitzer; V Taylor; U Suter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glia     Volume:  30     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-06-09     Completed Date:  2000-06-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  290-300     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Institute of Cell Biology, Swiss Federal Institute of Technology, Zurich, Switzerland.
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MeSH Terms
Brain-Derived Neurotrophic Factor / pharmacology
Cell Differentiation
Cells, Cultured
Embryo, Mammalian
Neurotrophin 3 / metabolism*
Platelet-Derived Growth Factor / metabolism*
Protein-Tyrosine Kinases / metabolism
RNA, Messenger / analysis
Rats, Inbred Strains
Receptor, Nerve Growth Factor / analysis
Receptor, Platelet-Derived Growth Factor alpha / genetics
Receptor, Platelet-Derived Growth Factor beta / genetics
Receptor, trkC / genetics
Schwann Cells / drug effects*,  metabolism*
Stem Cells / drug effects*,  metabolism*
Time Factors
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Neurotrophin 3; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger; 0/Receptor, Nerve Growth Factor; 0/platelet-derived growth factor BB; EC Kinases; EC, Platelet-Derived Growth Factor alpha; EC, Platelet-Derived Growth Factor beta; EC, trkC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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