Document Detail


Platelet concentrates from fresh or overnight-stored blood, an international study.
MedLine Citation:
PMID:  21223294     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
BACKGROUND: Whole blood and also buffy coats (BCs) can be held for a few hours or overnight before processing into blood components or platelet concentrates (PCs). Individual studies have reported a range of outcomes regarding in vitro variables for PCs prepared from fresh and stored whole blood. In this multicenter study, effects of storage of whole blood or BCs on the in vitro quality of PCs were studied.
STUDY DESIGN AND METHODS: The leukoreduced BC PCs were prepared from fresh BCs (2-8 hr after collection; fresh/fresh), from BCs at 20 to 24 hours after collection (fresh/stored), or from BCs prepared from whole blood stored for 20 to 24 hours (stored/fresh). PCs were stored on a flat-bed shaker at 20 to 24°C for 7 days. PCs were tested on Days 0 (only fresh/fresh), 1, 5, and 7 for in vitro quality. There were six participating centers that tested all three conditions with n = 6 per condition.
RESULTS: In comparison to fresh/stored and stored/fresh PCs, fresh/fresh PCs exhibited a lower platelet (PLT) count (Day 1-220 × 10(9) ± 70 × 10(9) vs. 324 × 10(9) ± 50 × 10(9) and 368 × 10(9) ± 56 × 10(9) PLTs/PC), lactate, pCO(2), and hypotonic shock response (HSR; Days 5 and 7; Day 7-50 ± 13% vs. 57 ± 12 and 63 ± 11%) and a higher pH, glucose, pO(2), and CD62P expression (than stored/fresh PCs only; Day 7-33 ± 10% vs. 28 ± 12 and 24 ± 11%; p < 0.05). No differences were observed for volume, swirling effect, white blood cell count, annexin V binding, or aggregation between these conditions.
CONCLUSION: Based on PLT count, HSR, and PLT activation, PCs are best prepared after 20 to 24 hours hold of the whole blood or BCs.
Authors:
M J Dijkstra-Tiekstra; P F van der Meer; R Cardigan; D Devine; C Prowse; P Sandgren; J de Wildt-Eggen;
Related Documents :
21283874 - A fully automated, multistep flow synthesis of 5-amino-4-cyano-1,2,3-triazoles.
21899494 - To mix or not to mix venous blood samples collected in vacuum tubes?
22234684 - Solulin increases clot stability in whole blood from humans and dogs with hemophilia.
2848724 - Renaturation of guanidine-unfolded tryptophan synthase by multi-mixing stopped-flow dil...
1464564 - The influence of loud sound on red blood cell velocity and blood vessel diameter in the...
22417834 - Fentanyl: toxic or therapeutic? postmortem and antemortem blood concentrations after tr...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  51 Suppl 1     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  38S-44S     Citation Subset:  IM    
Copyright Information:
© 2010 American Association of Blood Banks.
Affiliation:
Sanquin Blood Bank North East Region, Groningen, the Netherlands. m.dijkstra@sanquin.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Improving the predictive value of red blood cell storage trials: lessons from the Biomedical Excelle...
Next Document:  Evaluation of the overnight hold of whole blood at room temperature, before component processing: pl...