Document Detail

Platelet and aorta arachidonic and eicosapentaenoic acid levels and in vitro eicosanoid production in rats fed high-fat diets.
MedLine Citation:
PMID:  8827696     Owner:  NLM     Status:  MEDLINE    
There is a significant interest in the interrelationship between long-chain n-3 and n-6 fatty acids due to their ability to modulate eicosanoid production. In general, the intake of arachidonic acid (AA) results in enhanced eicosanoid production, whereas n-3 polyunsaturated fatty acids (PUFA) decrease the production of eicosanoids from AA. The purpose of this study was to investigate whether the effects of dietary AA on eicosanoid production in the rat were correlated with the AA and EPA levels in platelets and aorta (eicosanoid-producing tissues). Four groups of male Sprague-Dawley rats were fed a high-fat diet enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (approximately 100 mg/day of EPA + DHA) for 24 d. During the last 10 d, the four groups were orally supplemented with 0, 30, 60, and 90 mg/day of ethyl arachidonate. A further group of rats was fed a control diet (without long-chain n-3 PUFA) for 24 d. In vitro aorta prostacyclin (PGI2) production, serum thromboxane A2 (TxA2) production and plasma, and platelet and aorta phospholipid (PL) fatty acids were measured. Enriching the diet with n-3 PUFA resulted in significant reductions in tissue AA levels and an increase in the n-3 PUFA, particularly EPA. On this diet, the AA to EPA ratio was 1:1 in platelet PL, and it was 2:1 in the aorta PL. There were significant decreases in the in vitro PGI2 and TxA2 production compared with the control animals. The inclusion of AA in the diet resulted in marked increases in AA levels in the platelet and aorta PL with corresponding decreases in EPA. The lowest dose of AA (30 mg/rat) reversed the effects of 100 mg/day of n-3 PUFA on AA levels in platelet and aortic PL and on in vitro aorta PGI2 and serum TxA2 production. The dietary AA caused a differential (twofold) increase in TxA2 relative to PGI2 for all three levels of AA supplementation. There were greater changes in the levels of AA and/or EPA in platelet PL compared with the aorta PL, which might have accounted for the differential effects of these PUFA on thromboxane production compared with PGI2 production in this study.
A J Sanigorski; A J Sinclair; T Hamazaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  31     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-12-17     Completed Date:  1996-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  729-35     Citation Subset:  IM    
School of Human Nutrition and Public Health, Deakin University, Geelong, Victoria, Australia.
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MeSH Terms
Animal Feed
Aorta / chemistry*,  metabolism
Arachidonic Acids / administration & dosage,  blood*,  metabolism
Blood Platelets / chemistry*
Dietary Fats / administration & dosage*,  metabolism
Eicosanoids / biosynthesis*
Eicosapentaenoic Acid / administration & dosage,  blood*,  metabolism
Epoprostenol / biosynthesis
Fatty Acids, Omega-3 / administration & dosage
Fatty Acids, Unsaturated / chemistry,  metabolism*
Phospholipids / analysis,  blood
Random Allocation
Rats, Sprague-Dawley
Thromboxane A2 / biosynthesis,  blood
Reg. No./Substance:
0/Arachidonic Acids; 0/Dietary Fats; 0/Eicosanoids; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Unsaturated; 0/Phospholipids; 1553-41-9/Eicosapentaenoic Acid; 35121-78-9/Epoprostenol; 57576-52-0/Thromboxane A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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